There have been conflicting results regarding a correlation between CD133 expression and disease outcome. To assess CD133 expression in patients with acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) and to evaluate its correlation with the different clinical and laboratory data as well as its relation to disease outcome, the present study included 60 newly diagnosed acute leukemic patients; 30 ALL patients with a male to female ratio of 1.5:1 and their ages ranged from 9 months to 48 years, and 30 AML patients with a male to female ratio of 1:1 and their ages ranged from 17 to 66 years. Flow cytometric assessment of CD133 expression was performed on blast cells. In ALL, no correlations were elicited between CD133 expression and some monoclonal antibodies, but in AML group, there was a significant positive correlation between CD133 and HLA-DR, CD3, CD7 and TDT, CD13 and CD34. In ALL group, patients with negative CD133 expression achieved complete remission more than patients with positive CD133 expression. In AML group, there was no statistically significant association found between positive CD133 expression and treatment outcome. The Kaplan-Meier curve illustrated a high significant negative correlation between CD133 expression and the overall survival of the AML patients. CD133 expression is an independent prognostic factor in acute leukemia, especially ALL patients and its expression could characterize a group of acute leukemic patients with higher resistance to standard chemotherapy and relapse. CD133 expression was highly associated with poor prognosis in acute leukemic patients.

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Preeclampsia (PE) is a medical condition characterized by hypertension and proteinuria in pregnant females following 20 weeks of gestation. It is the most common cause of fetal morbidity and mortality, which has no curative therapeutic strategy, except for delivery of the placenta. The association between PE and aberrant microRNA (miRNA) expression in placentas was first reported in 2007 and a number of other similar findings have since been demonstrated.The aim of this study was to assess the level of expression of miR-155 in Egyptian women with preeclampsia and its role in pathogenesis and prognosis of the disease. This study was a case control study included a total of 40 pregnant females; 9 cases with early onset PEdiagnosed < 34 weeks of gestation whose mean age was 32.4 years, and 21 case of late onset PEdiagnosed ≥ 34 weeks of gestation whose mean age was 32.2 years admitted to the obstetrics and gynaecology department, Banha University Hospitals, in addition to 10 normotensive pregnant women of matched age as a control group. MIRNA 155 expression level were in PE cases when compared to control groups (p<0.001). MIRNA 155 expression level showed no significant difference between early onset and late onset PE. miRNA 155 expression showed significantly higher level in PE than normotensive pregnant females with no difference between early and late onset PE.

Objective: This study aims to assess the level of miR-210 in Egyptian women with pre-eclampsia (PE) and to evaluate its role in diagnosis and prognosis of the disease. Subjects and Methods: The study was conducted on 30 pregnant women with PE divided into two groups: group (I): included 15 cases with mild PE and group (II): included 15 cases of severe PE and, 20 healthy pregnant women with matched age and sex were included as control group. All women included in the study were subjected to, history taking full clinical examination, laboratory investigations included (CBC), (PT), (ALT, AST), (urea, creatinine), detection of protein in urine, as well as miRNA-210 gene expression by RT-PCR. Results: Patients with PE showed a highly significantly increase in serum miR-210 (P value <0.001) compared to control as well as, it was higher in severe PE than in mild PE (P value <0.001). MiR 210 have highly significant positive correlation with (systolic, diastolic, MABP), (Proteinuria) and (PT) a significant positive correlation with (AST), (ALT),.However no significant correlation was found with (Hb%) ,platelet count, PTT and INR, serum urea and creatinine. PTT was statistically significant (P value 0.002).The best cutoff value of PE (2.03) with sensitivity and specificity of 90.0% and 85.0% respectively. Conclusion: Expression of mir-210 is upregulated in pre-eclampsia and was higher in severe than in mild. Hence, the serum miR-210 can be used as a diagnostic, prognostic biomarker in PE patients and understanding pathophysiology.