Objective: This study aims to assess the level of miR-210 in Egyptian women with pre-eclampsia (PE) and to evaluate its role in diagnosis and prognosis of the disease. Subjects and Methods: The study was conducted on 30 pregnant women with PE divided into two groups: group (I): included 15 cases with mild PE and group (II): included 15 cases of severe PE and, 20 healthy pregnant women with matched age and sex were included as control group. All women included in the study were subjected to, history taking full clinical examination, laboratory investigations included (CBC), (PT), (ALT, AST), (urea, creatinine), detection of protein in urine, as well as miRNA-210 gene expression by RT-PCR. Results: Patients with PE showed a highly significantly increase in serum miR-210 (P value <0.001) compared to control as well as, it was higher in severe PE than in mild PE (P value <0.001). MiR 210 have highly significant positive correlation with (systolic, diastolic, MABP), (Proteinuria) and (PT) a significant positive correlation with (AST), (ALT),.However no significant correlation was found with (Hb%) ,platelet count, PTT and INR, serum urea and creatinine. PTT was statistically significant (P value 0.002).The best cutoff value of PE (2.03) with sensitivity and specificity of 90.0% and 85.0% respectively. Conclusion: Expression of mir-210 is upregulated in pre-eclampsia and was higher in severe than in mild. Hence, the serum miR-210 can be used as a diagnostic, prognostic biomarker in PE patients and understanding pathophysiology.
Background: Immune thrombocytopenia (ITP) is an autoimmune disease with excessive platelet destruction and reduced platelet production. Progranulin (PGRN) has a protective effect against autoimmune diseases by competitive binding tumor necrosis factor-α receptors. Thus, we assessed the serum progranulin level in relation to platelet count in ITP patients, pre-and post-induction of corticosteroid therapy. Methods: A casecontrol study was conducted on 30 ITP patients and 30 apparently healthy subjects serving as a control group. Platelet count and serum progranulin levels were measured in newly diagnosed ITP patients before and 3−7 days after starting corticosteroid treatment. Results: The mean serum level of PGRN was significantly higher in ITP patients pretreatment compared to healthy control subjects (P <0.001). After 3-7 days of treatment of ITP patients with corticosteroid; the platelets count increased significantly, whereas serum PGRN level decreased significantly (P <0.001 each). Serum PRGN showed a significant negative correlation with platelets count pre-steroid treatment and a significant positive correlation with platelets count post-steroid treatment. Conclusion:The elevated serum PGRN levels in ITP patients supports its involvement in the disease pathogenesis. The inversely correlated serum PGRN levels with platelets count in ITP patients before and after treatment induction suggest that PGRN may be a useful biomarker that helps the diagnosis, treatment decisions and follow up of ITP patients.
Toxemia is a genuine entanglement of pregnancy, which is the reason for 60,000 maternal passings every year around the world. Notwithstanding the notable maternal hazard factors, for example, hypertension, diabetes mellitus, antiphospholipid neutralizer condition, heftiness, maturing, and various pregnancies, ongoing investigations have distinguished the job of hereditary and immunological elements in the pathogenesis of toxemia. PE could prompt maternal and pre-birth dismalness. Imbalanced cytokine arrange and modified degrees of a few fiery and calming cytokines have been accounted for in PE The examination intended to evaluate the serum levels of IL-4, IL-17 and IL-35 in toxemia patients well as in normotensive pregnant women.Blood tests were gathered from 25 toxemia patients and 15 normotensive pregnant ladies. Serum levels of IL-4, IL-17 and IL-35 in totally examined bunches were controlled by an immunoenzyrnatic test. The outcomes indicated higher groupings of IL-17 in PE and huge contrast between PE cases and control gathering and no noteworthy distinction in IL4 and IL35. A critical positive relationship was found between serum IL-17 and negative connection with maternal age, gravidity state, SBP and DBP.
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