A B S T R A C T Ectomycorrhizal fungi, Scleroderma spp., is potential to promote the growth of seedlings for forestry plants. This research explored the Scleroderma spp., from rhizosphere of Fagaceae in School of Biology forest, investigated the compatibility of Scleroderma spp., with Lithocarpus urceolaris seedlings and studied its effectiveness. The result showed that there were three species of Scleroderma: Scleroderma sinnamariense, Scleroderma columnare and Scleroderma citrinum. Lithocarpus urceolaris inoculated with Scleroderma sinnamariense, resulted in the highest growth of plants (56.55 cm) compared to S. columnare, S. citrinum and control. Diameters of seedlings inoculated with the three species of Scleroderma did not show significant different but they were significant different from control. The three species of Scleroderma had the same growth of colonizations (30%) classified as middle colonizations. There were changes in morphology and anatomy of roots from the infection of three species of Scleroderma. Mantle was clearly observed to cover the root surface and the mycelia formed the Hartig net. There was compatibility between L. urceolaris and three species of Scleroderma. It is suggested that inoculating these Scleroderma to L. urceolaris is necessary to increase the quality of growth seedling.
The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
Research on the antibacterial potency of fresh extract from leaves of Jamaican cherry (Muntingia calabura L.) in inhibiting the growth of Shigella dysenteriae had been conducted in the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The study aimed to determine the potential of fresh extract from Jamaican cherry leaves in inhibiting the growth of S. dysenteriae and to determine its Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against S. dysenteriae. The results showed that the fresh extract of Jamaican cherry leaves was potent as an antimicrobial agent against pathogenic bacteria S. dysenteriae. It was shown through 12 - 14 mm diameter of inhibition zone which was classified as strong inhibition. The MIC was measured at 3.125% while MBC was undetermined. According to these findings, it can be concluded that the fresh extract from Jamaican cherry leaves was potent to inhibit the growth of S.dysentriae at 3.125% concentration, yet unable to kill it.
Antibiotics are metabolites of microorganisms that can kill or inhibit the growth of other microorganisms. This study aims to increase the inhibition of antibiotics produced from endophytic BES-5 positive Gram bacteria. The increasing of antibiotic activity is carried out by optimizing the production of antibiotics from bacteria. Antibiotic activity was determined by the Kirby method with E. coli as test bacteria. The results showed to obtain antibiotics with very strong category characteristics, with optimum conditions for incubation temperature of 33 0 C; pH of production media 7.5; agitation of 150 rpm; 5% inoculum; 3% soaking corn water as inducer, 5% inoculum, 0.5% glucose as carbon source. The optimum condition besides increasing the inhibition also accelerates the production of antibiotics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.