Improving Antibiotic Activities From Endophytic Isolate Bes-5 Gram Positive Bacteria

Abstract

Antibiotics are metabolites of microorganisms that can kill or inhibit the growth of other microorganisms. This study aims to increase the inhibition of antibiotics produced from endophytic BES-5 positive Gram bacteria. The increasing of antibiotic activity is carried out by optimizing the production of antibiotics from bacteria. Antibiotic activity was determined by the Kirby method with E. coli as test bacteria. The results showed to obtain antibiotics with very strong category characteristics, with optimum conditions for incubation temperature of 33 0 C; pH of production media 7.5; agitation of 150 rpm; 5% inoculum; 3% soaking corn water as inducer, 5% inoculum, 0.5% glucose as carbon source. The optimum condition besides increasing the inhibition also accelerates the production of antibiotics.