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BACTERIOLOGICAL QUALITY OF WASHED AND UNWASHED EGGS STORED UNDER ROOM TEMPERATURE AND REFRIGERATION AND CONTAMINATED WITH Pseudomonas aeruginosa ABSTRACTThe aim of this study was to evaluate the effect of sanitization and storage temperature on the quality of commercial eggs inoculated with Pseudomonas aeruginosa. We used 240 large eggs, without cracks, from Dekalb White laying hens at 30 to 40 weeks of age. The experimental design used was two blocks in a 2 x 2 factorial arrange (washed/non-washed and refrigerated/non-refrigerated) with twelve replicates. The eggs were contaminated by handling, with 1.5 x 10 5 colony-forming units (CFU) of Pseudomonas aeruginosa and stored at 5 °C and 25 °C for 30 days. Each ten days the eggshell and contents were submitted to bacteriological analyses. Variance analyses were performed and the data were compared by Tukey test. The results showed that Pseudomonas aeruginosa were isolated from the shells and contents of all eggs. Therefore, when the eggs were sanitized and stored at 5 ºC the contamination was less intense. The correlation between the presences of Pseudomonas aeruginosa in the shell and contents was high, when the eggs were not sanitized nor refrigerated. The conclusion of this study was that the egg must be sanitized and refrigerated when the stored for more than 30 days.

Introduction: Hemophagocytic lymphohistiocytosis (HLH) is a condition characterized by a hyperinflammatory state and persistent macrophage activation, resulting in reactive phagocytosis of the hematopoietic elements. In children, it is usually a hereditary disorder, while in adults it is usually acquired secondary to viral infections, collagenoses, or tumors. Although accounting for 10% of hematologic malignancies, HLH is rarely associated with multiple myeloma (MM) and other plasmacytic dyscrasias. Patient concerns: A 64-year-old Brazilian man seeked medical care with a 3-month history of intermittent fever, weight loss, night sweats, and progressive anemic symptoms. Diagnosis: Total blood count showed severe bicytopenia (normocytic-normochromic anemia and thrombocytopenia), biochemical exams showed elevation of creatinine, as well as monoclonal peak in serum protein electrophoresis, high IgA dosage, and serum immunofixation with IgA kappa paraprotein. Bone marrow biopsy showed 30% of monoclonal and phenotypically anomalous plasmocytes, confirming the diagnosis of MM. Diagnosis of HLH was established by the presence of clinical and laboratory criteria: fever, splenomegaly, cytopenias, hypofibrinogenemia, hyperferritinemia, elevation of triglycerides, and several figures of erythrophagocytosis in bone marrow aspirate. Interventions: The patient experienced pulse therapy with methylprednisolone for hemophagocytic lymphohistiocytosis, followed by initial therapy for multiple myeloma with cyclophosphamide and dexamethasone. Outcomes: Once the diagnosis of MM and secondary hemophagocytic syndrome was established, the patient had a rapid clinical deterioration despite the established therapeutic measures, evolving with cardiovascular failure, acute liver failure, acute disseminated intravascular coagulation, worsening renal dysfunction requiring dialysis support, respiratory dysfunction, and lowering of consciousness, characterizing rapid multiple organ dysfunction, ultimately leading to the death of the patient. Innovation: Here, we aimed to describe the sixth reported case of HLH associated with MM, according to cases cataloged in the PubMed database, and the first case evaluated by 18-fluordeoxyglucose positron emission tomography (18-FDG-PETCT). Conclusion: Our case report seeks to provide support for a better clinical and laboratory characterization of this rare paraneoplastic entity associated with MM, and aims to call the attention of hematologists and intensivists to this condition that falls within the scope of the differential diagnosis of rapid onset multiple organ failure in patients with plasmacytic neoplasms.

-The objective of this study to verify the physicochemical quality of commercial washed and unwashed eggs, experimentally inoculated on the shell with Pseudomonas aeruginosa and stored at 5 and 25 o C for 30 days. A total of 384 eggs, classified as large, from light Dekalb White laying hens at 30 to 40 weeks of age, were used. The experimental design consisted of two blocks in a 2 × 2 × 2 factorial arrangement (contamination, washing, and refrigeration) with six replicates. The sanitization was performed by mechanical washing (hot water with chlorhexidine 20% and 8% active content). Eggs were contaminated by handling with 1.5 × 10 5 colony-forming units (cfu) of Pseudomonas aeruginosa/mL solution, and stored at 5 and 25 o C for 30 days. Each ten days, analyses of the eggs were carried out, for the assessment of physical (egg weight, specific gravity, shell thickness, yolk, albumen and shell percentage, Haugh unit, yolk and albumen rates) and chemical (albumen and yolk pH) characteristics. There were interactions between sanitization, storage temperature and contamination. The cooling process maintained the egg internal quality even when there was contamination on the shell by Pseudomonas aeruginosa inoculum. Cooling slows down the weight loss and promotes better internal physical and chemical quality of the eggs during the 30 days of storage regardless of the contamination and washing processes.

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This report regards a 40-year-old man, diagnosed with sickle cell anaemia (SCA; HbSS), who was admitted to our emergency service with symptoms of vaso-occlusive crisis. The patient was not under chronic transfusion therapy, and the baseline haemoglobin was 7 g/dL. He was initially treated with opioid analgesics and hydration. The initial laboratory investigation showed Hb = 7.1 g/dL, Ht = 21.1%, lactate dehydrogenase (LDH) = 384 U/L, total bilirubin = 2.3 mg/dL and indirect bilirubin = 1.32 mg/dL. Anti-E and anti-Di a were identified in the antibody screening using the gel method (ID-DiaPanel and ID-DiaPanel-P, BIORAD, Lagoa Santa, Brazil). Previous immunohaematological investigation had shown only anti-E. He was transfused with two red blood cell (RBC) units (254 and 261 mL), without any clinical intercurrences during the infusion. The patient's phenotype was R 0 r; K−; Jk(a + b−); Fy(a + b−); S−, s+ and Dia−, and the transfused units had the same phenotype. In addition, molecular investigation for the most common RHD 1 and RHCE variants (Restriction Fragment Length Polymorphism-RFLP-PCR designed to detect c.733C>G) was performed and was negative. All transfused units were HbS-negative, and the patient's blood volume was 4.5 L. The laboratory tests performed approximately 8 hours after the transfusion revealed a sharp decrease in the haemoglobin levels (posttransfusion Hb = 5 g/dL) and an increase in LDH (550 U/L). Parvovirus polymerase chain reaction was performed and was negative.

RESUMO Objetivou-se com este estudo verificar a qualidade físico-química (pH) e bacteriológica da água destinada ao consumo dos moradores do assentamento de Canudos localizado na região de Palmeiras de Goiás. Foram coletadas 60 amostras da água utilizada pelos moradores do assentamento, as quais foram realizadas aleatoriamente, em cada propriedade, aplicou-se paralelamente um questionário buscando informações sobre o destino da água, limpeza dos poços e animais criados na propriedade. Observou-se a presença de Escherichia coli, Aeromonas e Pseudomonas sp. na água que abastece o assentamento. O pH da água esteve dentro dos padrões de normalidades exigido pelo Ministério da saúde. Concluiu-se que a maioria dos moradores do assentamento realizam a limpeza dos poços anualmente. A água destinada para o consumo das famílias também é destinada para consumo dos animais e manutenção de hortaliças cultivadas nas propriedades.

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