Objective: Aristolochia albida Duch is frequently used by alternative medicine to treat some pathologies like hepatitis. Our main objective was to evaluate the acute oral toxicity of the ethanolic extract of this plant.Methods: Exploratory tests for acute oral toxicity are performed in vivo on Wistar albino rats in a limit test of 2000 mg/kg for 14 d in accordance with the OECD Guidelines 423. The clinical signs were observed every day, followed by measurement of body weight change, the haematological and biochemical examinations were executed and statistical analysis was performed.
Results:The various clinical signs observed after administration and for 14 d were recorded and no mortality was observed. With the exception of white blood cells, mean cell volume and platelets with statistically significant difference in control (p<0.05), all the haematological parameters showed an insignificant statistical difference to the control ratio (p>0.05). For biochemical parameters, except blood glucose and total protein of control batches, which show a significant statistical difference after 14 d (p>0.05), all the biochemical parameters show the statistically insignificant difference for test and controls batches (p>0.05) as well as the weight variation of the animals.
Conclusion:The ethanolic extract of the leaves of A. albida Duch (EEAr) had not toxic effect on the biochemical and hematological parameters studied at a dose of 2000 mg/kg. The lethal dose is therefore over 2000 mg/kg.
Objective: To do the phytochemical screening and then to measure total phenols, condensed tannins, flavonoids of aqueous, hydroethanolic and ethanolic extracts of Aristolochia albida Duch. in order to identify the best preparation technique and extraction solvent. The antiradical activity of the different extracts of this plant were evaluated to determine the extract that has the best anti-radical activity. Methodology and Results: Aristolochia albida Duch. is a medicinal plant from the Aristolochiaceae family, widely used in traditional medicine in Benin. In this work ethanolic, hydroethanolic and aqueous extract were prepared from the leaves of this plant. The quantitative estimation of total phenols, tannins and flavonoids by the colorimetric method showed that the extracts are rich in these compounds. Evaluation of antioxidant power was performed using the method of DPPH free radical trapping. The result indicated that The ethanolic extract of Aristolochia albida (IC50 = 0.23 mg/ml, IC50 = Concentration inhibiting 50% of reaction) showed more antioxidant and anti-radical capacity compared to hydroethanolic extract (IC50 = 0, 62 mg/ml) and the aqueous extract (IC50 = 0. 65 mg/ml).
Conclusion and application of results:Overall, there is a correlation between anti-radical powers and phenolic contents phytoconstituents (polyphenols, flavonoids, tannins) extracts of the plant studied.
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