RESUMO:Neste estudo, uma técnica de cromatografia líquida de alta resolução em fase reversa (CLAE-FR) para a determinação de ácido rosmarínico em Cordia verbenacea foi desenvolvida e validada. A análise de regressão foi avaliada, com observação de uma boa linearidade (r = 0,9997). Os valores obtidos para a precisão e exatidão estão de acordo com as diretrizes do ICH e com a legislação brasileira. Os valores de repetibilidade e precisão intermediária foram 2,79% e 4,76%, respectivamente. Os limites de detecção e de quantificação de ácido rosmarínico foram de 1,92 µg/mL e 5,81 µg/mL, respectivamente. Os resultados mostraram que o método desenvolvido é uma técnica por CLAE-FR de confiança para a determinação de ácido rosmarínico em tintura de C. verbenacea. Além disso, essa metodologia foi aplicada em estudo sazonal, que revela uma correlação positiva relativamente forte entre o período de chuvas e o teor de ácido rosmarínico. Palavras-chave: Cordia verbenacea, ácido rosmarínico, CLAE.ABSTRACT: Determination of rosmarinic acid in Cordia verbenacea by liquid chromatography: applicability in seasonal study. In this study, a reverse phase-high performance liquid chromatography (RP-HPLC) technique for determination of rosmarinic acid in the Cordia verbenacea was developed and validated. A regression analysis was performed, with the observation of good linearity (r =0.999949). The values obtained for precision and accuracy determination are in agreement with ICH guidelines and the Brazilian legislation. The values of repeatability and intermediate precision were 2.79% and 4.76%, respectively. The detection and the quantitation limits of the rosmarinic acid were 1.92 µg/mL and 5.81 µg/ mL, respectively. The results demonstrated that the developed method is a reliable RP-HPLC technique for the determination of rosmarinic acid in C. verbenacea tincture. In addition, this methodology was applied at a seasonal study indicating relatively strong positive correlation between the rain period and the rosmarinic acid content.
This study aims to evaluate the microbial contamination of Mikania sp. (plant drug, tincture and syrup) and Cordia verbenacea (plant drug, tincture and ointment). Furthermore, it had been investigated the possible interference caused by secondary metabolites from these species on proposed evaluation. The microbial contamination was determined using the pour plate method. The evaluation of the interference in the process of the microbiological quality was performed by determining the recovery of viable microorganisms using Escherichia coli (ATCC 25922) and Candida albicans (ATCC 90028). In the evaluation the microbiological quality, dried leaves from C. verbenacea and Mikania sp. showed 4.5 x 104 CFU/g and 2.08 x 104 CFU/g of bacteria, respectively. Regarding count of C. verbenacea tincture, was found 3.3 x 103 CFU/mL for yeasts and molds and 2.2 x 103 CFU/ mL for bacteria. For the ointment containing C. verbenacea tincture was found 1.1 x 103 CFU/g of bacteria, while fungi were not detected. Regarding microbial contamination of the syrup was not observed growth of bacteria, molds and yeasts in any of repetitions. Also, derivatives from both species were able to inhibit the growth of strains of E. coli and C. albicans. Thus, the presence of secondary metabolites with antimicrobial activity on C. verbenacea and Mikania sp. seems to interfere in the evaluation of the microbiological quality of products derived from plant drug.
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