The objective of this study was to evaluate biological and phytochemical properties of the aqueous extract from the leaves of Miconia chamissois Naudin (AEMC). Phytochemical properties were assessed by analyzing the chromatographic profile and the polyphenol content of AEMC. Biological properties evaluation was conducted based on cytotoxicity assay and by evaluating the antioxidant, antimicrobial, and enzymatic inhibition activities. Results indicated the presence of phytochemicals in AEMC such as flavonoids and polyphenols, including rutin, isoquercitrin and vitexin derivatives. AEMC showed antioxidant activity, which may be attributed to the high polyphenolic content. Moreover, AEMC demonstrated in vitro enzyme inhibition activity against tyrosinase and alpha-amylase, as well as showed low cytotoxicity. On the other hand, AEMC exhibited weak antimicrobial activity against S. aureus and C. albicans. Thus, AEMC is a promising alternative in search of potential drugs for the treatment of diseases induced by oxidative stress and inflammation, conditions due to hyperpigmentation processes, such as melisma, as well as for diabetes.
This study aims to evaluate the microbial contamination of Mikania sp. (plant drug, tincture and syrup) and Cordia verbenacea (plant drug, tincture and ointment). Furthermore, it had been investigated the possible interference caused by secondary metabolites from these species on proposed evaluation. The microbial contamination was determined using the pour plate method. The evaluation of the interference in the process of the microbiological quality was performed by determining the recovery of viable microorganisms using Escherichia coli (ATCC 25922) and Candida albicans (ATCC 90028). In the evaluation the microbiological quality, dried leaves from C. verbenacea and Mikania sp. showed 4.5 x 104 CFU/g and 2.08 x 104 CFU/g of bacteria, respectively. Regarding count of C. verbenacea tincture, was found 3.3 x 103 CFU/mL for yeasts and molds and 2.2 x 103 CFU/ mL for bacteria. For the ointment containing C. verbenacea tincture was found 1.1 x 103 CFU/g of bacteria, while fungi were not detected. Regarding microbial contamination of the syrup was not observed growth of bacteria, molds and yeasts in any of repetitions. Also, derivatives from both species were able to inhibit the growth of strains of E. coli and C. albicans. Thus, the presence of secondary metabolites with antimicrobial activity on C. verbenacea and Mikania sp. seems to interfere in the evaluation of the microbiological quality of products derived from plant drug.
Agradeço primeiramente a Deus pelo dom da vida e por ter acordado todos os dias desses dois anos com muita saúde. Agradeço a Ele pela oportunidade e por todas as bênçãos a mim concedidas desde sempre. Agradeço também à Professora Yris Maria Fonseca-Bazzo, por me aceitar como aluna, me apoiar e me orientar. Muito obrigada pela ajuda e dedicação e espero que Deus possa abençoá-la e retribuir sua generosidade. Agradeço aos meu pais, Maria de Lourdes e Lucídio, pelo encorajamento e suporte durante esse perídio, pelos conselhos e por não me deixar desanimar. Espero deixa-los mais orgulhosos. Muito obrigada ao meu irmão, Lucas, por me dar exemplos diários de superação e, mesmo sem entender muito bem, sempre me apoiou e demonstrou carinho do seu jeito. Muito obrigada à Professora Pérola de Oliveira Magalhães pela empolgação, suporte e por mover montanhas por nós, seus alunos. À Professora Dâmaris Silveira pela imensa ajuda e orientação. Ao Professor Christopher Fagg pela disponibilidade, bom humor e por me ajudar no reconhecimento e na coleta da espécie vegetal. À Doutora Paula Souza por aceitar meu convite e fazer parte da banca examinadora e por me ajudar sempre. À professora Yanna Nóbrega e ao seus alunos do Laboratório de Pesquisas em Doenças Imunogenéticas e Crônico-degenerativas e à professora Eliete Guerra do Laboratório de Histopatologia Bucal pela ajuda com os ensaios envolvendo células.
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