Aim: We carried out this research to assess thiol disulfide balance in patients with early-stage endometrial cancer. Methods: Fifty-seven endometrial cancer patients and 60 age-matched healthy subjects volunteered for this study. Thiol disulfide parameters and the ratios of these parameters were examined using a colorimetric system. We also evaluated total antioxidant capacity, total oxidant capacity and oxidative stress index. Results: Subjects with endometrial cancer had statistically significantly lower serum native thiol and total thiol levels (224.2 [122.8-267.5] and 270.5 [171.6-323.2], respectively) than healthy subjects (281.35 [213.45-358.9] and 339. 55 [274.1-425.95], respectively) (P = 0.001, P < 0.001). Subjects with endometrial cancer had statistically significantly higher serum disulfide/native thiol and disulfide/total thiol ratios (12.22 [8.77-17.61] and 9.82 [7.46-13.02], respectively) than healthy subjects (8.9 [6.79-16.35] and 7.36 [5.9-12.32], respectively) (P = 0.038, P = 0.028). Disulfide/native thiol ratio appeared to be strongly and positively correlated with the stage of endometrial cancer (r = 0.827, P < 0.001). Conclusion: This is an initial report related to thiol disulfide balance in endometrial cancer patients. We believe that oxidative stress contributes both to the evolution and to the progression of the disease. We conclude that deterioration of thiol disulfide balance due to oxidative stress is likely to contribute to the etiopathogenesis of endometrial cancer.
Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.
Aim: The evaluation of dynamic thiol-disulfide homeostasis among patients with the cancer of the uterine cervix. Methods: The study was conducted in 62 cervical cancer patients and 61 healthy women who had been followed up in an obstetrics and gynecology clinic between September 2018 and April 2020. Serum disulfide, native thiol, total thiol, ischemia modified-albumin, total antioxidant and oxidant capacities, and oxidative stress index values were measured in all participants. Results: The mean plasma disulfide levels of the cervical cancer group was statistically significantly higher than that of the control group (25.79 AE 6.90 μmol/L, 22.31 AE 6.11 μmol/L, respectively) (P = 0.004). Plasma native thiol and total thiol levels were lower in cervical cancer patients (299.27 AE 99.05 μmol/L and 350.86 AE 102.72 μmol/L, respectively) compared to controls, but no statistically significant difference was observed (318.00 AE 93.75 μmol/L and 376.44 AE 98.51 μmol/L, respectively) (P = 0.284, P = 0.161). With respect to the ischemia modified-albumin level, no statistically significant difference was observed between two groups. There were statistically significant positive association between disulfide level and both the stage of cervical cancer (r = 0.278, P = 0.029) and total oxidant capacity level (r = 0.256, P = 0.046). Conclusion: Dynamic thiol-disulfide homeostasis may participate in the pathophysiological mechanisms of cervical cancer and may be a potential biomarker for early identification of cervical cancer in future.
Amaç: Araştırma gebe kadınların doğum korkularını ve bu korkuları etkileyen faktörlerini belirlemek amacıyla tanımlayıcı ve ilişki arayıcı tasarımda yapılmıştır. Gereç ve Yöntemler: Çalışma, Muğla Sıtkı Koçman Üniversitesi Eğitim ve Araştırma Hastanesi’nin kadın hastalıkları ve doğum polikliniğinde gerçekleştirilmiştir. Çalışmaya 203 gebe dahil edilmiştir. Verilerin toplanmasında gebelerin sosyodemografik özelliklerini belirleyen tanılama formu ve doğum korkusunu değerlendiren Wijma Doğum Beklentisi/ Deneyimi Ölçeği- A Versiyonu kullanılmıştır. Veriler SPSS 20,0 programında tanımlayıcı istatistikler, Student t-testi, Ki-Kare ve Mann Whitney U analizleri ile değerlendirilmiştir. Bulgular: Katılımcıların yaş ortalaması 28,36±4,85 olup, %42,8'inin üniversite mezunu ve %64,9'unun gelir düzeyi gider düzeyine eşit olduğu saptanmıştır. Araştırmada gebelerin doğum korkusu puan ortalamasının 52,48±15,70 olduğu belirlenmiştir. Gebelerin bulundukları trimester dönemleri, gebelikte eşinden destek alma, daha önce gebelik geçirme durumları ile doğum korkusu puan ortalamaları arasında anlamlı bir ilişki bulunmuştur. Bununla birlikte canlı doğum sayısı, önceki doğum şekli ve doğum öncesi gebelik ve doğum ile ilgili bilgi alma durumları arasında Wijma Doğum Beklentisi/ Deneyimi Ölçeği puan ortalamaları arasındaki ilişki bulunamamıştır. Sonuç: Araştırmada, gebelerin doğum korkusu düzeyleri yüksek bulunmuştur. Yaşı daha genç olan, eğitim seviyesi yüksek, geliri giderinden az olduğunu ifade eden, gebelerde de doğum korkusu yüksek, ilk gebeliği olan, eşinden fiziksel ya da duygusal destek alamadığını ifade eden ve ikinci trimesterde olan kadınların doğum korkusunun daha yüksek olduğu belirlenmiştir. Ayrıca çalışmada sağlık profesyonelleri tarafından verilen eğitim ile kadınların doğum korkusunun azaldığı saptanmıştır.
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