Assessment of thiol disulfide balance in early‐stage endometrial cancer

Abstract

Aim: We carried out this research to assess thiol disulfide balance in patients with early-stage endometrial cancer. Methods: Fifty-seven endometrial cancer patients and 60 age-matched healthy subjects volunteered for this study. Thiol disulfide parameters and the ratios of these parameters were examined using a colorimetric system. We also evaluated total antioxidant capacity, total oxidant capacity and oxidative stress index. Results: Subjects with endometrial cancer had statistically significantly lower serum native thiol and total thiol levels (224.2 [122.8-267.5] and 270.5 [171.6-323.2], respectively) than healthy subjects (281.35 [213.45-358.9] and 339. 55 [274.1-425.95], respectively) (P = 0.001, P < 0.001). Subjects with endometrial cancer had statistically significantly higher serum disulfide/native thiol and disulfide/total thiol ratios (12.22 [8.77-17.61] and 9.82 [7.46-13.02], respectively) than healthy subjects (8.9 [6.79-16.35] and 7.36 [5.9-12.32], respectively) (P = 0.038, P = 0.028). Disulfide/native thiol ratio appeared to be strongly and positively correlated with the stage of endometrial cancer (r = 0.827, P < 0.001). Conclusion: This is an initial report related to thiol disulfide balance in endometrial cancer patients. We believe that oxidative stress contributes both to the evolution and to the progression of the disease. We conclude that deterioration of thiol disulfide balance due to oxidative stress is likely to contribute to the etiopathogenesis of endometrial cancer.

Thiol‐disulfide status of patients with cervical cancer

“…In the study of endometrial cancer, it was stated that the ratios of oxidants and antioxidants may be useful in demonstrating oxidative stress. 8 The ratio of oxidants to antioxidants (disulfide/native thiol ratio) was increased in patients with breast and endometrial cancers similar to patients in our study. We may conclude that dynamic TD homeostasis is in the direction of oxidation in patients with cervical cancer as in patients with endometrial and breast cancers.…”

“…In the study of endometrial cancer, it was reported by Sezgin et al that as the rate of disulfide/native thiol increased, the depth of myometrial invasion also increased. 8 Our study showed that the levels of disulfide were found to be progressively increased as the stage of the cervical cancer progressed. The evolving of this oxidative environment is probably due to the effect of HPV E6/7 oncoproteins to increase reactive oxygen species in cellular metabolism.…”

“…17 Oncological studies using dynamic TD hemostasis by evaluating the total body thiol pool are also available as in our study (basal cell carcinoma, prostate, breast, lung, endometrium cancer). 8,[18][19][20][21] Meanwhile, our study has the broadest population study that evaluates dynamic TD homeostasis in malignant cases. Among these cancers, total thiol and native thiol levels which have antioxidant properties were found to be lower in prostate, breast, lung and endometrium cancers than those in the control group.…”

“…Among these cancers, total thiol and native thiol levels which have antioxidant properties were found to be lower in prostate, breast, lung and endometrium cancers than those in the control group. 8,[19][20][21] These four types of cancer incidentally show rapid progression. In contrast, it was found high in basal cell carcinoma, which shows slow progression.…”

“…Details of blood samples retrieval and storage, determination of TD, TAC, TOC and OSI parameters have been described in detail in our previous publication. 8 IMA levels were analyzed according to the method established by Bar-Or et al 12 The results were articulated as IU/mL.…”