Evaluation of Dynamic Thiol-Disulfide Balance in Preinvasive Lesions of the Cervix

Abstract

Purpose: This study aimed to determine the potential clinical use of dynamic thiol disulfide balance in cases with preinvasive lesions of the cervix.Methods: One hundred and sixteen patients with high-grade squamous intraepithelial lesion, one hundred patients with low-grade squamous intraepithelial lesion and one hundred and ten healthy controls were enrolled in the study. A fully automated colorimetric system was used to determine the levels of thiol-disulfide parameters. The ischemia-modified albumin, total oxidant-antioxidant capacity, oxidative stress index of the retrieved cases were further analysed.Results: Native thiol and total thiol levels are significantly lower in the high-grade squamous intraepithelial lesion group according to control group (p:0.004 and p:0.015, respectively). Disulfide level is significantly increased in the high-grade squamous intraepithelial lesion group compared to control group (p:0.004). Oxidative stress index levels in high-grade squamous intraepithelial lesion group were observed as significantly higher according to the control group (p:0.014). Ischemia-modified albumin levels in the high-grade squamous intraepithelial lesion group were observed as significantly higher compared to the control group (p:0.020). Disulfide levels are positively correlated with risk type of Human papillomavirus (r:0.420, p<0.001).Conclusion: The analysis of dynamic thiol disulfide balance revealed considerable oxidative damage in patients with Human papillomavirus -related cervical precursor lesions compared to women with ordinary cytology specimens. Therefore, investigation of thiol disulfide balance with presented method represents a new promising test for early diagnosis and management of women at high risk for cervical cancer.

Impact of psoriasis disease and its treatment with Etanercept on serum and saliva thiol-disulfide homeostasis

“…The mechanism of how this elevated thiol levels act is not clear, but it might be through its responsibility of the increased keratinocyte proliferation in the psoriasis disease [42]. The present study results measured an increase in blood disulfide levels indicating that a shift in thiol/disulfide imbalance is significant in psoriasis thus causes a highly significant increased oxidative stress as a result of tissue inflammation [39]. Disulfide bridges play an important role in maintaining the stability of mature keratin [47].…”

“…The formed disulfides are converted back into thiols by the cleavage of S-S bonds [37,38]. The Dynamic thiol/ disulfide homeostasis is responsible for many processes such as antioxidant defense, regulation of enzyme functions, signal transduction, apoptosis, and other processes [19] and the alteration in the thiol/disulfide balance appears to be involved in the pathogenesis of many diseases, especially diseases that are characterized by chronic inflammation [39][40][41].…”

Thiol-Disulfide Homeostasis in Skin Diseases

“…Therefore, for example, HPV E6 decreases the level of enzymatic antioxidants SOD and GPX and enhances ROS production [ 150 ]. HPV helps infected cells adapt to a pro-oxidant microenvironment [ 21 ]. Low molecular weight thiols have many functions in the human body, including antioxidant defense, cell signaling and modulation of the immune response [ 151 ].…”

“…Based on these findings, elevated serum thiol levels may represent a mechanism for blocking free thiols in the HPV structure [ 44 ]. Changes in the thiol-disulfide balance have been detected in premalignant and malignant lesions caused by HPV [ 21 , 152 ]. Regarding warts, we identified two studies that evaluated TDH.…”

“…[ 19 ]. The alteration of the thiol-disulfide balance appears to be involved in the pathogenesis of many diseases, especially diseases characterized by chronic inflammation [ 21 , 22 , 23 ].…”