The degradation of phenolics and anthocyanins was observed after bioaccessibility tests. © 2017 Society of Chemical Industry.

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The protein extraction from the blue-green microalgae Spirulina platensis was carried out using ultrasound-assisted extraction and response surface methodology (RSM) was used to optimise extraction conditions. Extraction yield, total phenolic content, antioxidant activity and in vitro protein digestibility of protein extracts were determined. A three factors Box-Behnken design (BBD) of experiments was employed at pH values of 7, 8 and 9; temperatures of 25, 35, and 45°C; and for durations of 60, 90 and 120 minutes. Based on the RSM analysis, optimum extraction conditions (temperature 45°C, pH 7.46 and time 120 min) were obtained for extraction yield (29.05%), total phenolic content (3.52 mg caffeic acid equivalent/g dw), antioxidant activity (11.32 mg Trolox equivalent/g dw) and in vitro protein digestibility (99.36%). We report the first evaluation of the in vitro protein digestibility of Spirulina platensis and find it to be over 90%. This value is higher than the in vitro protein digestibility values of proteins obtained from other algae and plant species, and, in particular, is greater than that of commercial soybean protein isolate.

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Deep eutectic solvents are applied as a new type of sustainable solvents.The new generation of green solvents are considerable utilize to extraction of bioactive compounds due to their physicochemical properties and structure. In this study, the extraction process of phenolic compounds from rhubarb roots using of ultrasound-assisted extraction (UAE) method was investigated.Totally 8 different types of choline chloride-and L-proline-based deep eutectic solvents containing various H-bond donors/acceptors were synthesized.The extraction conditions for phenolic acids such as effect of different DESs,extraction temperature,water content in nades,ratio of solid to liquid and extraction time were studied.The optimal parameters were found to be extraction time of 30 min, temperature of 313.15 K, DES 30 wt % water and solid/liquid ratio of 1:20 led to significantly effect on the extraction of phenolic contents from plant material. The quantification and characterization of two biactive flavonoids in the extracts was analyzed via ultra-high pressure liquid chromatography (UPLC) with photodiode dedection.Compared with conventional extraction system, deep eutectic solvents (DESs) showed high extraction efficiency for both polar and less polar compounds. This study reveals,the developed DESs have significant as alternative new green dissolving agents in the extraction of phenolic compounds from plant resource due to their high solubilization ability and low toxicity.