Application of Choline Chloride as Natural Deep Eutectic Solvents for the Green Extraction of Phenolic Compounds from Rheum Ribes Leaves

Abstract

Deep eutectic solvents are applied as a new type of sustainable solvents.The new generation of green solvents are considerable utilize to extraction of bioactive compounds due to their physicochemical properties and structure. In this study, the extraction process of phenolic compounds from rhubarb roots using of ultrasound-assisted extraction (UAE) method was investigated.Totally 8 different types of choline chloride-and L-proline-based deep eutectic solvents containing various H-bond donors/acceptors were synthesized.The extraction conditions for phenolic acids such as effect of different DESs,extraction temperature,water content in nades,ratio of solid to liquid and extraction time were studied.The optimal parameters were found to be extraction time of 30 min, temperature of 313.15 K, DES 30 wt % water and solid/liquid ratio of 1:20 led to significantly effect on the extraction of phenolic contents from plant material. The quantification and characterization of two biactive flavonoids in the extracts was analyzed via ultra-high pressure liquid chromatography (UPLC) with photodiode dedection.Compared with conventional extraction system, deep eutectic solvents (DESs) showed high extraction efficiency for both polar and less polar compounds. This study reveals,the developed DESs have significant as alternative new green dissolving agents in the extraction of phenolic compounds from plant resource due to their high solubilization ability and low toxicity.

Enhanced Total Phenolic Content Extraction From Cucumis Melo L. (Kultik) Kernel by Deep Eutectic Solvent (Des)

“…The same result, the decrease in the yield after 50 0 , was also demonstrated by previous studies [20,23,36,37]. However, Bildik [6] and Zhou et. al.…”

“…The reason why the TPC value was on the decrease as extraction temperature was on the increase after a certain extent could be due to lower mass transfer [16]. In addition, extraction conditions led to the difference in the study of [6] and [42] which used ultrasound-assisted extraction. Zhou et.…”

“…The extraction was performed with DES (70% DES (1:4 molar ratio of ChCI:EtGl) + 30% water), which was determined as the best molar ratio to extract phenolic compounds in the earlier part of this study. Different extraction times (0, 15, 30, 45, and 60 min) were used to compare the effect of extraction time on extraction efficiency at 30 0 C, which was chosen by the author according to [6]. Then, the effect of extraction temperature on TPC extraction was evaluated by using various temperatures (30 0 C, 50 0 C, 60 0 C, and 80 0 C) in 30 min, which was determined to be optimal in the earlier part of this study.…”

“…A ceaseless demand rises to find a green solvent, deemed as an alternative to conventional solvents [5]. Many recent studies showed that deep eutectic solvents (DES) could be a good option for conventional solvents [6,21,30,34]. DESs are mainly formed by mixing hydrogen bond acceptors (HBA) and hydrogen bond donors (HBD).…”

Morphological Characterization of Nicotiana tabacum Inflorescences and Chemical-Functional Analysis of Extracts Obtained from Its Powder by Using Green Solvents (NaDESs)

“…The order of efficiency in the extraction of the TF from the powdered flowers was as follows CU = EW > AW = DW > FGS = SALA > LAS > CAP ( Table 3 ). The efficacy of CU was previously reported for flavonoids, i.e., quercetin and kaempferol [ 39 ]. Previously, the presence of flavonoids was reported in corolla or petals and calyxes or sepals of tobacco flower, using organic solvents for its extraction, namely isopropyl alcohol-water (1:1, v / v ), followed by solvents which progressively increased in alcohol content [ 12 ].…”