BackgroundIncreasing natural drug demand for pharmaceutical uses has encouraged scientifics all over the world to explore medicinal plants recognized as efficient remedies. In this context, extracted oil from pumpkin seeds (Cucurbita pepo L.) is an interesting target, as it is composed with prominent pharmacological properties to possible wound healing treatments.MethodsThe composition and content of certain bioactive constituents of the cold pressed oil obtained from pumpkin seeds (Cucurbita pepo L.) were analyzed and studied for their wound healing properties. Uniform wounds were induced on the dorsum of 18 rats, randomly divided into three groups. The wounds were photographed, and topically treated with saline solution (control group), 0.13 mg/mm2 of a reference drug (“Cicaflora cream®”), and 0.52 μl/mm2 of pumpkin’s oil each 2 days until the first group is completely healing and so far biopsies were histologically assessed.ResultsThe composition and content of tocopherols, fatty acids, and phytosterols were determined. The results showed an excellent quality of pumpkin oil with high content of polyunsaturated fatty acids (Linoleic acid: 50.88 ± 0.106 g/100 g of total fatty acids), tocopherols (280 ppm) and sterols (2086.5 ± 19.092 ppm). High content of these bioactive components were in agreement with an efficient wound healing by the mean of an in vivo study. In fact, morphometric assessment and histological findings revealed healed biopsies from pumpkin oil treated group of rats, unlike untreated group, and a full re-epithelialization with reappearance of skin appendages and well organized collagen fibers without inflammatory cells.ConclusionsThis study showed the significance of oil from pumpkin seeds (Cucurbita pepo L.) as a promising drug to healing wounds in animal assays. As a whole, pumpkin’s oil would be recommended in the nutritional and medicinal purposes.

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The effects of untreated and treated olive mill wastewater on seed germination, plant growth and soil fertility were studied. Tomato (Lycopersicon esculentum), chickpea (Cicer arietinum), bean (Vicia faba), wheat (Triticum durum) and barley (Hordeum vulgare) were tested for the germination index and growth in soil irrigated by olive mill wastewater. Lepidium sativum was used as the standard species for the germination index and phytotoxicity evaluation. We measured soil properties, crop growth, herbage biomass, leaf elongation and productivity. The results show beneficial effects using treated olive mill wastewater. The treated plants showed an improvement in seed biomass, spike number, plant growth, and a similar or even better dry productivity than plants irrigated with water, e.g. Hordeum vulgare 102%, Triticum durum 105%, Cicer arietinum 101% and Vicia faba 102%. An increase in the germination index from 100% to 115% was observed. Soil organic matter, respiration and enzymatic activities were also enhanced by treated olive mill wastewater ferti-irrigation. However, even diluted 10 times, untreated olive mill wastewater inhibited the species germination, e.g. 1.2% for Hordeum vulgare and 40% for Cicer arietinum and Vicia faba, and plant growth (16-42.5%). Leaf necrosis and low productivity were observed in crops ferti-irrigated by untreated olive mill wastewater. olive mill wastewater / germination index / phytotoxicity / crop growth / soil fertility