Systemic chemotherapy has lost its position to treat cancer over the past years mainly due to drug resistance, side effects, and limited survival ratio. Among a plethora of local drug delivery systems to solve this issue, the combinatorial strategy of chemo-hyperthermia has recently received attention. Herein we developed a magneto-thermal nanocarrier consisted of superparamagnetic iron oxide nanoparticles (SPIONs) coated by a blend formulation of a three-block copolymer Pluronic F127 and F68 on the oleic acid (OA) in which Curcumin as a natural and chemical anti-cancer agent was loaded. The subsequent nanocarrier SPION@OA-F127/F68-Cur was designed with a controlled gelation temperature of the shell, which could consequently control the release of curcumin. The release was systematically studied as a function of temperature and pH, via response surface methodology (RSM). The bone tumor killing efficacy of the released curcumin from the carrier in combination with the hyperthermia was studied on MG-63 osteosarcoma cells through Alamar blue assay, live-dead staining and apoptosis caspase 3/7 activation kit. It was found that the shrinkage of the F127/F68 layer stimulated by elevated temperature in an alternative magnetic field caused the curcumin release. Although the maximum release concentration and cell death took place at 45 °C, treatment at 41 °C was chosen as the optimum condition due to considerable cell apoptosis and lower side effects of mild hyperthermia. The cell metabolic activity results confirmed the synergistic effects of curcumin and hyperthermia in killing MG-63 osteosarcoma cells.
The
complex reconstructive surgeries for which patient-specific
orthopedic, maxillofacial, or dental implants are used often necessitate
wounds that are open for a considerable amount of time. Unsurprisingly,
this allows bacteria to establish implant-associated infection, despite
the scrupulous sterilization efforts made during surgery. Here, we
developed a prophylactic bactericidal coating via electrophoretic
deposition technology for two 3D-printed porous titanium implant designs.
The surface characteristics, antibiotic release behavior, antibacterial
properties, and impact on osteoblast cell proliferation of the optimized
coatings were investigated. The results unequivocally confirmed the
biofunctionality of the implants in vitro. This study reveals a new
avenue for future antibacterial patient-specific implants.
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