Proteins have short half-life because of enzymatic cleavage. Here, a new protein nanocarrier made of graphene oxide (GO) + Chitosan (CS) is proposed to successfully prevent proteolysis in protein and simultaneously retain its activity. Bovine serum albumin (BSA) and collagenase were loaded on GO and GO-CS to explore the stability and activity of proteins. SEM, AFM, TEM, DSC, UV-Vis, FT-IR, RBS, Raman, SDS-PAGE and zymography were utilized as characterization techniques. The protecting role of GO and GO-CS against enzymatic cleavage was probed by protease digestion analysis on BSA, where the protease solution was introduced to GO-BSA and GO-CS-BSA at 37 °C for 0.5-1-3-6 hours. Characterizations showed the successful synthesis of few layers of GO and the coverage by CS. According to gelatin zymographic analysis, the loaded collagenase on GO and GO-CS lysed the gelatin and created non-staining bands which confirmed the activity of loaded collagenase. SDS-PAGE analysis revealed no significant change in the intact protein in the GO-BSA and GO-CS-BSA solution after 30-minute and 1-hour exposure to protease; however, free BSA was completely digested after 1 hour. After 6 hours, intact proteins were detected in GO-BSA and GO-CS-BSA solutions, while no intact protein was detected in the free BSA solution.

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Collaboration between pharmacists and general practitioners (GPs) has been shown to enhance patient care and outcomes. The aim of the present study was to investigate the collaborative working relationship between pharmacists and GPs in terms of their attitudes, role perceptions, experience with collaborative practice, preferred method of communication, areas of current and further collaboration, and perceived barriers to interprofessional collaboration in a sample of the Iranian population. We distributed 318 questionnaires to community pharmacists and GPs in Tehran. Both groups had a positive attitude towards collaboration; however, about half the respondents reported only occasional collaborative practice. Both groups preferred communication by telephone or face-toface communication by fax or letter. Few current areas of collaboration were identified; however, an area favoured by both groups was "decision-making for patients' pharmacotherapy". The two groups expressed concern about possible fragmentation of patient care with the involvement of multiple health care providers, and perceived lack of face-to-face communication as a possible barrier to collaboration. Les deux groupes étaient favorables à la collaboration, mais près de la moitié des participants ont rapporté n'entretenir des relations de collaboration que sur une base occasionnelle. Les deux groupes ont déclaré préférer une communication par téléphone ou en face à face que par fax ou courrier. Peu de domaines faisant l'objet d'une collaboration actuelle ont été identifiés. Cependant, les deux groupes avaient pour domaine de prédilection « la prise de décision concernant la pharmacothérapie des patients ». Les deux groupes se sont dit préoccupés par une possible fragmentation des soins dispensés aux patients du fait de l'apparition de multiples prestataires de soins de santé, et percevaient le manque de communication en face à face comme une barrière potentielle à la collaboration. ‫اإلسالمية‬

: Graphene derivatives (GDs) have captured the interest and imagination of pharmaceutical scientists. This review exclusively provides pharmacokinetics and pharmacodynamics information with a special focus on biopharmaceuticals. GDs can be applied as multipurpose pharmaceutical delivery systems due to their ultra-high surface area, flexibility, and fast mobility of charge carriers. Improved effects, targeted delivery to tissues, controlled release profiles, visualization of biodistribution and clearance, and overcoming drug resistance are examples of the benefits of GDs. This review also focused on the application of GDs for the delivery of biopharmaceuticals and some challenges on the way to the commercialization of GDs were discussed.

Background. The dental pulp is a heterogeneous soft tissue that supplies nutrients and acts as a biosensor to identify pathogenic stimuli. Regeneration of the dental pulp is one of the desirable topics for researchers. Graphene oxide nanosheets (nGOs) help overexpression of the genes related to odontogenic differentiation of stem cells from dental pulps and increases attachment and proliferation of dental pulp stem cells. Despite its benefits, nGO may be considered as a threat to the environment and human health. Therefore, the purpose of this study was to evaluate the biocompatibility potential of graphene oxide (nGO), chitosan functionalized graphene oxide (nGO-CS), and carboxylated graphene (nGO-COOH) when exposed to human dental pulp stem cells (hDPSCs). Material and Methods. Some different aspects of biocompatibility of nGO, nGO-CS, and nGO-COOH were synthesized, and several intracellular effects induced by different concentrations of graphene-based nanosheets, including cell viability, intracellular oxidative damages, and various factors such as LDH, GSH, SOD, MDA, and MMP, were studied on hDPSCs. Results. According to results, IC50 was determined as 232.01, 467.81, and ≥1000 μg/mL for nGO, nGO-CS, and nGO-COOH, respectively. These results demonstrated the lower toxicity and higher cytocompatibility of nGO-CS and nGO-COOH compared to nGO. nGO-COOH not only has any adverse effect on the cell membrane and mitochondrial activity but also shows slight antioxidant activity at some concentrations. Conclusion. The findings help design safe and cytocompatible nGO derivatives for biomedical applications in dental fields.