Background/aim: To investigate the relative frequency of biopsied nonneoplastic oral mucosal lesions in Ankara, Turkey. Materials and methods:Biopsy records of a single center from 2000-2012 were retrospectively collected. Diagnosis was recorded and evaluated with respect to patient demographics (age, sex) and location of the lesion. Results:Of 11,980 biopsies, 1732 (14.5%) were mucosal nonneoplastic lesions. Hyperplastic lesions (n = 1000, 57.7%) with fibroepithelial hyperplasia in 30.9% of patients were the most common type of oral nonneoplastic lesions. The mean age of patients differed with respect to type of mucosal lesion, tending to be lower in patients with reactive lesions. Dermatoses showed a female predominance. Conclusion:Our findings revealed that hyperplastic lesions were the most common among nonneoplastic oral mucosa lesions. Geographic and ethnic differences of patients with various types of oral mucosal lesions require further investigation.

Purpose:The aim of this retrospective study is to determine the radiological features of keratocystic odontogenic tumors (KCOT) using cone-beam computed tomography (CBCT).Materials and Methods:CBCT images of 28 patients who had histopathologically-confirmed KCOT were retrospectively reviewed from the archives of the Department of Dentomaxillofacial Radiology, Gazi University Faculty of Dentistry. The location and size of KCOT, cortical expansion, cortical perforation, relation with the impacted teeth, and the impact on the mandibular canal were evaluated.Results:The mean age of patients at initial diagnosis was 34.5 years. Patients with an impacted tooth were significantly younger than those without an impacted tooth (p<0.05). Among KCOTs, 21% were localized in the maxilla whereas 79% were found in the mandible. The lesions localized in the mandible were mostly found in the retromolar-ramus region. Of these patients, 93% had cortical expansion and 75% had bone perforation. Of the 22 mandibular lesions, 20 were in close proximity of the mandibular canal and 18 (90%) of these lesions had caused destruction in the mandibular canal.Conclusion:KCOTs exhibit their aggressive nature in the jaw bone. CBCT is a useful radiological imaging method to examine the radiologic characteristics of KCOTs such as bone destruction and their spatial relations with the neighboring anatomic structures.

Objective: KRAS mutation attenuates treatment effect of cetuximab. The purpose of this study was to develop a rapid and specific method to detect KRAS mutation at codon 12 using novel gene amplification methods.Study Design: Previously, loop-mediated isothermal amplification (LAMP) assay was developed to amplify copies of particular DNA sequence. LAMP assay is rapid and specific method rather than polymerase chain reaction process. We modified LAMP assay by using peptide nucleic acid (PNA) to suppress an amplification of wild-type allele.Results: We successfully developed modified LAMP assay in several cultured cell lines. By using this assay, we can amplify DNA fragment of mutated KRAS gene within 20 minutes. Primers bracketing the nucleotides of KRAS codon 12 were designed so that all possible alleles would be amplified by this assay.Conclusions: The modified LAMP assay is a rapid, and highly sensitive detection assay for cancer mutations.

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