Multiple Large Peripheral Giant Cell Granuloma: A Case Report

Abstract

Objective: KRAS mutation attenuates treatment effect of cetuximab. The purpose of this study was to develop a rapid and specific method to detect KRAS mutation at codon 12 using novel gene amplification methods.Study Design: Previously, loop-mediated isothermal amplification (LAMP) assay was developed to amplify copies of particular DNA sequence. LAMP assay is rapid and specific method rather than polymerase chain reaction process. We modified LAMP assay by using peptide nucleic acid (PNA) to suppress an amplification of wild-type allele.Results: We successfully developed modified LAMP assay in several cultured cell lines. By using this assay, we can amplify DNA fragment of mutated KRAS gene within 20 minutes. Primers bracketing the nucleotides of KRAS codon 12 were designed so that all possible alleles would be amplified by this assay.Conclusions: The modified LAMP assay is a rapid, and highly sensitive detection assay for cancer mutations.

Peripheral Giant Cell Granuloma in Maxilla: Case Report

“…PGCGs are exophytic lesions occurring in the oral cavity. These lesions grow from the periodontal ligament or periodontium due to local irritants such as tartar, plaque, incompatible restoration, tooth extraction, and chronic inflammation (9,10). In this case report are presented the diagnosis and treatment of PGCG in the right maxilla caused by chronic trauma.…”

Mandibulada Periferal Dev Hücreli Granülom: Olgu Sunumu

“…Periferal Dev Hücreli Granülomlar oral kavitede meydana gelen ekzofitik lezyonlardır. Bu lezyonlar tartar, plak, uyumsuz restorasyon, diş çekimi, kronik enflamasyon gibi lokal iritanlara bağlı olarak periodontal ligament ya da periodonsiyumdan kaynak alarak gelişir (10,11). Lezyonun tüm yaş gruplarını etkilemekle beraber 40-60 yaş arası grupta yoğunlaştığı ve mandibulanın maksilladan daha sık etkilendiği görülmüştür (9).…”