Human samples of Human Herpesvirus 6A were collected during 13 October 2021 to 15 of December 2021. Human Herpesvirus 6A was including (15-49years). The real time polymerase chain reaction (RT-qPCR) method was detected of all samples, the results showed fifty five(55%) positive cases while (45%) negative cases. The Population groups studied samples subject groups were distribution into (4) groups including (15-25, 26-36 and 37-47 and 48-58) year, changed age too gender. The second groups (26-36 years) were high cases of human infected (43.6%) in compare of aged groups then [37-47 (32.7%); 15-25 (14.5%); 48-58 (9%) years]. The percentage of males(54.5%) is higher than females (45.5%) . the number of positive cases in infertility patients with 5months,1-5 years of infertility was(27)cases more than that in an infertility patients with 6-10years of infertility was(18)cases and at least in an infertility patients with 11-15 years of infertility was(10)cases. Regarding the sequences test, the results showed the percentage of similarity with the studied strains at a rate ranging between (96.63-93.64%) as shown in the table (2), two samples were selected based on the concentration of the virus, cycles, different age groups, gender in addition to the sample source areas at the time of collection.
Aims: Diagnosing the Coronavirus and viruses that share infection for different age groups through real time PCR, as well as using the Sanger sequencing method to homology sequence identity between local human Coronavirus isolate and NCBI-BLAST submitted Coronavirus isolate.
Study Design: Cross section.
Place and Duration of Study: From the central health laboratory and quarantine centers, work was carried out in different places for the purpose of diagnosis in Al-Hakim and Al-Sadr Teaching Hospital and the Central Health Center in addition to the private laboratories, between April 2020 and July 2021.
Methodology: Diagnosing the virus through qRT- PCR, PCR and sanger sequencing. Sample: We included 300 patients (174 men, 128 women; age range 18- 83years) with from people who were infected for the first time with the Coronavirus and repeated infections with the virus with other viruses that affect the respiratory system.
Results: The current study showed that the number of cases of Coronavirus infection (110) case, Influenza virus (90) case, Parainfluenzavirus (65) case, Metapneumovirus (108)case and Rhinovirus (95)case for the period from 4-4-2020 up to 26-26 7-2021 all viruses were diagnosed through qReal time PCR technique by designing primers according to the location NCBI Regarding the sequences test, the results showed the percentage of similarity with the studied strains at a rate ranging between (99.92 - 78 %).
Conclusion: Most of the infections with the Coronavirus are common with respiratory viruses in different proportions, in addition to the fact that the age group (51-61) year is more infected, and (110) case of Influenza shares with the Coronavirus more infection and the results of Sanger sequences between(99.92 - 78 %).
The study was conducted on seventy individuals of both genders who have been exposed to human cytomegalovirus, a common illness in Iraq. Total cases of human cytomegvirus associated with malignant brain tumors were detected by a real time PCR technique. This resulted in only thirty-six cases of true infection. Of these 24 cases were female, while 12 cases of male infected. The titer to assay the presence of anti- and pro-inflammatories was assessed in sera of all patients by using ELISA kits to evaluate cytokines. This indicated that the pro-inflammatory IL12, after seven days increased (1.67±0.23 pg/ml), while IL4, an anti-inflammatory, decreased to reach (0.39±0.16 pg/ml) (at p<0.05) in the plasm of the experimental patients compared with the control group.
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