Diagnosis of human herpesvirus 6A association infertility by RT-qPCR and detection sanger sequencing

Abstract

Human samples of Human Herpesvirus 6A were collected during 13 October 2021 to 15 of December 2021. Human Herpesvirus 6A was including (15-49years). The real time polymerase chain reaction (RT-qPCR) method was detected of all samples, the results showed fifty five(55%) positive cases while (45%) negative cases. The Population groups studied samples subject groups were distribution into (4) groups including (15-25, 26-36 and 37-47 and 48-58) year, changed age too gender. The second groups (26-36 years) were high cases of human infected (43.6%) in compare of aged groups then [37-47 (32.7%); 15-25 (14.5%); 48-58 (9%) years]. The percentage of males(54.5%) is higher than females (45.5%) . the number of positive cases in infertility patients with 5months,1-5 years of infertility was(27)cases more than that in an infertility patients with 6-10years of infertility was(18)cases and at least in an infertility patients with 11-15 years of infertility was(10)cases. Regarding the sequences test, the results showed the percentage of similarity with the studied strains at a rate ranging between (96.63-93.64%) as shown in the table (2), two samples were selected based on the concentration of the virus, cycles, different age groups, gender in addition to the sample source areas at the time of collection.