Sequences of S-surface of Human COVID-19

Abstract

Aims: Diagnosing the Coronavirus and viruses that share infection for different age groups through real time PCR, as well as using the Sanger sequencing method to homology sequence identity between local human Coronavirus isolate and NCBI-BLAST submitted Coronavirus isolate.
 Study Design: Cross section.
 Place and Duration of Study: From the central health laboratory and quarantine centers, work was carried out in different places for the purpose of diagnosis in Al-Hakim and Al-Sadr Teaching Hospital and the Central Health Center in addition to the private laboratories, between April 2020 and July 2021.
 Methodology: Diagnosing the virus through qRT- PCR, PCR and sanger sequencing. Sample: We included 300 patients (174 men, 128 women; age range 18- 83years) with from people who were infected for the first time with the Coronavirus and repeated infections with the virus with other viruses that affect the respiratory system.
 Results: The current study showed that the number of cases of Coronavirus infection (110) case, Influenza virus (90) case, Parainfluenzavirus (65) case, Metapneumovirus (108)case and Rhinovirus (95)case for the period from 4-4-2020 up to 26-26 7-2021 all viruses were diagnosed through qReal time PCR technique by designing primers according to the location NCBI Regarding the sequences test, the results showed the percentage of similarity with the studied strains at a rate ranging between (99.92 - 78 %).
 Conclusion: Most of the infections with the Coronavirus are common with respiratory viruses in different proportions, in addition to the fact that the age group (51-61) year is more infected, and (110) case of Influenza shares with the Coronavirus more infection and the results of Sanger sequences between(99.92 - 78 %).