The preparations and amino acid compositions of highly purified tropocollagen and insoluble polymerized collagen are described. These collagens appear to be very suitable for comparative studies in an investigation of the cross-linkages that are introduced into tropocollagen during the formation of polymerized collagen.
A topographical study of the articular surface of 21 femoral heads removed for surgery of osteoarthrosis was made using the scanning electron microscope. Sections of degenerate cartilage were pretreated with H2O2 and trypsin to reveal the collagenous framework. Torn and frayed collagen bundles were evident on all the specimens studied, the length of these bundles varying from 20 to 150 micron. The frequency of the bundles over the degenerate fibrous areas varied considerably, and bundle sizes ranged from 20 to 70 micron. The topography of a single femoral head removed from a rheumatoid arthritic was very much smoother, with torn fibre ends ruptured at the same level. Although the exposed bone was considerably "smoother" than the residual fibrocartilage, ruptured osteons and trabeculae revealed large voids in the surface which would presumably alter the lubrication and fluid flow characteristics of the functioning joint. The effects of these changes on joint tension and lubrication are discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.