The effect of chelating agents on collagen interfibrillar matrix interactions in connective tissue

Steven, F. S.

doi:10.1016/0005-2795(67)90526-0

Cited by 91 publications

(23 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Steven (24) showed more than 30 years ago that an EDTA solution can be used to release polymerized collagen from interfibrillar matrix of bovine or human tendons. The action of the chelating agent was rapid and reversible by the addition of calcium.…”

Section: Discussionmentioning

confidence: 99%

Ultrastructure of Achilles Tendons of Rats Treated with Ofloxacin and Fed a Normal or Magnesium-Deficient Diet

Shakibaei

Pfister

Schwabe

et al. 2000

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Fluoroquinolones can cause tendinitis and tendon rupture. However, toxicological as well as clinical information on quinolone-induced tendopathy is scarce. We performed extensive electron microscopic studies with Achilles tendon specimens from ofloxacin-treated rats. The drug was given at a dose of 1,200 mg/kg (body weight) orally. Juvenile Wistar rats received one or three oral doses each of 1,200 mg of ofloxacin/kg (body weight)/day. Three days after treatment, the tenocytes of their Achilles tendons showed degenerative alterations, such as multiple vacuoles and vesicles in the cytoplasm that had developed due to swellings and dilatations of cell organelles. Other indications of cell degradation were the occurrence of cell debris and cell detachment from the extracellular matrix accompanied by a loss of cell-matrix interaction. The tenocytes of juvenile Wistar rats that had been treated at day 36 with a single oral dose of 1,200 mg of ofloxacin/kg (body weight) and sacrificed either 3 or 6 months later exhibited similar degenerative alterations. The number of degenerative alterations of tenocytes after ofloxacin treatment was considerably higher in rats that had received a magnesium-deficient diet than in rats with normal magnesium status. Of the adult rats that had been treated once, 5 times, and 10 times with ofloxacin and killed 1 day later, only those with the 10-times treatment showed a significantly increased number of degeneratively altered tenocytes. In summary, effects observed in tendons show similar pathological features as described earlier in cartilage, indicating that quinolone-induced arthropathy and quinolone-induced tendopathy probably are different clinical manifestations of the same toxic effect on cellular components of connective tissue structures.Musculoskeletal adverse effects represent a small but significant fraction of the adverse effects observed during therapy with fluoroquinolones (0.5 to 2%). Besides arthralgia and myalgia, cases of tendinitis and tendon ruptures have also been described. As with quinolone-induced arthropathy, most cases of tendon disorders have occurred with pefloxacin. Tendinitis associated with other drugs such as ciprofloxacin, ofloxacin, norfloxacin, and enoxacin has been reported, but the incidence appears to be much lower. In trying to estimate the overall clinical significance of this effect, it should be kept in mind that probably quite a number of unidentified cases exist (2,3,22).Clinical information on quinolone-induced tendopathy is scarce. Selective clinical studies regarding this adverse effect have not been published, and toxicological data are very limited. Some studies in rats that have been published so far do not seem to reflect the clinical situation because the alterations are detectable in juvenile rats only and are preventable by dexamethasone (8-10).We performed extensive electron microscopic studies with Achilles tendon specimens from ofloxacin-treated rats. Our experiments were designed to answer several questions. First, are multiple do...

show abstract

Section: Discussionmentioning

confidence: 99%

Ultrastructure of Achilles Tendons of Rats Treated with Ofloxacin and Fed a Normal or Magnesium-Deficient Diet

Shakibaei

Pfister

Schwabe

et al. 2000

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The inhibition of destiffening caused by both inorganic and organic calcium channel blockers and by the intracellular chelator EGTA-AM suggests that the mechanical condition of the mesohyl is influenced by cellular processes i , yet it accelerated rather than retarded destiffening. The fact that collagen fibrils can be isolated from the mesohyl of C. reniformis by a medium containing EDTA (Garrone et al, 1975) indicates that, as in mammalian and echinoderm collagenous tissue (Dixon et al, 1972;Steven, 1967;Wilkie, 2005), divalent cations contribute directly to the cohesion of the mesohyl ECM. Therefore, it is possible that the effects of elevated [Ca 2+ ] and CaFASW alone are due to their direct influence on extracellular components of the mesohyl ECM and are not cell-mediated.…”

Section: Cellular Basis Of Variable Tensilitymentioning

confidence: 99%

Mechanical adaptability of a sponge extracellular matrix: evidence for cellular control of mesohyl stiffness inChondrosia reniformisNardo

Wilkie

Parma

Bonasoro

et al. 2006

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY The marine sponge Chondrosia reniformis Nardo consists largely of a collagenous tissue, the mesohyl, which confers a cartilaginous consistency on the whole animal. This investigation was prompted by the incidental observation that, despite a paucity of potentially contractile elements in the mesohyl, intact C. reniformis stiffen noticeably when touched. By measuring the deflection under gravity of beam-shaped tissue samples, it was demonstrated that the flexural stiffness of the mesohyl is altered by treatments that influence cellular activities, including [Ca2+]manipulation, inorganic and organic calcium channel-blockers and cell membrane disrupters, and that it is also sensitive to extracts of C. reniformis tissue that have been repeatedly frozen then thawed. Since the membrane disrupters and tissue extracts cause marked stiffening of mesohyl samples, it is hypothesised that cells in the mesohyl store a stiffening factor and that the physiologically controlled release of this factor is responsible for the touch-induced stiffening of intact animals.

show abstract

“…The tendon was cleaned of adhering fat and muscle, cut into small pieces, and thoroughly washed in 0.05 M Tris-HCl (pH 6.8) and distilled water. The purified fibre of tendon was centrifuged at 4˚C at 13,000ϫg for 15 min, and used as substrate (Steven, 1967). All these steps were performed at 4˚C.…”

Section: Methodsmentioning

confidence: 99%

The Solubilization of Unheated Cattle Achilles Tendon with Actinidin under Neutral and Acidic Conditions

Wada

Hosaka

Nakazawa

et al. 2004

FSTR

View full text Add to dashboard Cite

The solubilization of cattle achilles tendon with actinidin was investigated under neutral and acidic conditions. 1.43 to 1.92 and 0.97 to 3.19% of collagen were solubilized by treating the cattle achilles tendon with actinidin at 20˚C at pH 6.0 and 3.3, respectively. Furthermore, SDS-polyacrylamide gel electrophoresis of reaction mixtures with actinidin demonstrated that actinidin degraded the tendon into collagen subunit chain, ␤-and ␣-chain and peptide fragments of various sizes at 20˚C at pH 6.0 and 3.3. These results indicated that actinidin could solubilize the insoluble collagen in unheated cattle achilles tendon at 20˚C at pH 6.0 and 3.3 and that a large proportion of the resulting peptide fragments by actinidin seemed to be actinidin digests against elastin with a small contribution of hydroxyproline.

show abstract

The effect of chelating agents on collagen interfibrillar matrix interactions in connective tissue

Cited by 91 publications

References 8 publications

Ultrastructure of Achilles Tendons of Rats Treated with Ofloxacin and Fed a Normal or Magnesium-Deficient Diet

Ultrastructure of Achilles Tendons of Rats Treated with Ofloxacin and Fed a Normal or Magnesium-Deficient Diet

Mechanical adaptability of a sponge extracellular matrix: evidence for cellular control of mesohyl stiffness inChondrosia reniformisNardo

The Solubilization of Unheated Cattle Achilles Tendon with Actinidin under Neutral and Acidic Conditions

Contact Info

Product

Resources

About