Aggregation of fibrinogen molecules by metal ions

Steven, F. S.; Griffin, Margaret M.; Brown, B.S.; Hulley, T.P.

doi:10.1016/0141-8130(82)90071-x

Cited by 20 publications

(21 citation statements)

References 9 publications

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“…Following exposure to this metal, various changes were reported, such as alteration of several clotting factors activity (Loiseau et al, 1997), increase in platelet aggregation, reduction in clotting time, and increased expression of procoagulative genes and proteins (Gilmour et al, 2006). Other metals have also demonstrated procoagulant effects (Penglis and Michal, 1969;Maeda et al;Steven et al, 1982).…”

Section: Concentration (M) Whole Blood Coagulation Timementioning

confidence: 94%

Metals in air pollution particles decrease whole-blood coagulation time

Sangani

Soukup

Ghio³

2010

Inhalation Toxicology

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The mechanism underlying procoagulative effects of air pollution particle exposure is not known. The authors tested the postulate that (1) the water-soluble components of an air pollution particle could affect whole-blood coagulation time and (2) metals included in this fraction were responsible for this effect. Exposure to the water-soluble fraction of particulate matter (PM), at doses as low as 50 ng/ml original particle, significantly diminished the whole-blood coagulation time. Inclusion of deferoxamine prolonged coagulation time following the exposures to the water-soluble fraction, whereas equivalent doses of ferroxamine had no effect. Except for nickel, all metal sulfates shortened the whole-blood coagulation time. Iron and zinc were two metals with the greatest capacity to reduce the coagulation time, with an effect observed at 10 ng/ml. Finally, in contrast to the anticoagulants citrate and EDTA, their iron complexes were found to be procoagulative. The authors conclude that metals in the water-soluble fraction of air pollution particles decrease whole-blood coagulation time. These metals can potentially contribute to procoagulative effects observed following human exposures to air pollution particles.

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Section: Concentration (M) Whole Blood Coagulation Timementioning

confidence: 94%

Metals in air pollution particles decrease whole-blood coagulation time

Sangani

Soukup

Ghio³

2010

Inhalation Toxicology

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“…Thus it is conceivable that blood clotting is also sensitively controlled by hydration repulsion at the surfaces of the fibrinogen •y-chain. The importance of hydration repulsion at fibrinogen surfaces is apparent from the results that Cu 2+ and Zn 2+ (< 3 ITIM) caused fibrinogen to aggregate (pH 7-2) whereas aggregation of fibrinogen is not induced by Ca 2+ and Mg 2+ at the highest concentrations tested (0-O1 M; Steven et al 1982).…”

Section: Discussionmentioning

confidence: 99%

Effects of cations and anions on the rate of the acidic coagulation of casein micelles: the possible roles of different forces

Bringe

Kinsella

1991

Journal of Dairy Research

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Raw skim milk was diluted 1000-fold using distilled water or various salt solutions as specified. Smooth, hyperbolic profiles of coagulation rate v. pH for casein were calculated from recordings of turbidity (400 nm) with time. The effects of pH, cation type, anion type and cleavage of/c-casein by chymosin (EC 3.4.23.4) were determined. The maximum of pH-coagulation rate profiles decreased by 63, 85 and 94 % when the skim milk diluent was changed from water to salt solutions of NaCl (100 mM), CaCl 2 (50 mM) or MgCl 2 (50 mM). The maximum of the pH-coagulation rate profile was 15 times greater when the Ca salt was changed from CaCl 2 to Ca(SCN) 2 (50 mM). The highest pH at which casein coagulation occurred increased from 4 -45 to > 6*0 when Cu 2+ (1 mM) was included with casein micelles dispersed in CaCl 2 solution (50 mM). The addition of chymosin to casein micelles suspended in CaCl 2 solution (70 mM) eliminated the inhibition of casein coagulation by Ca 2+ at pH 4 -5. It is proposed that ions such as Mg 2+ , Ca 2+ , and Na + , which generally associate with casein phosphate and carboxylate groups, increased the H + concentration required to initiate the coagulation of casein, because H + must displace bound Ca 2+ , Mg 2+ or Na + to reduce repulsive hydration forces between casein micelles, allowing attractive hydration forces (e.g. hydrophobic phenomena) to cause casein coagulation. Furthermore, it is proposed that ions such as Cl~, Br~, N0 3~ and SCN~ bind to lysine, arginine and histidine groups and thereby decrease repulsive hydration forces between cationic casein micelles.

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“…Zinc‐induced precipitation is commonly used to fractionate proteins from complex mixtures such as human plasma 1–3. Recently, zinc has been used to precipitate purified human growth hormone as an initial step in preparing controlled‐release formulations of the protein in biodegradable microspheres 4, 5.…”

Section: Introductionmentioning

confidence: 99%

“…An industrially relevant protein precipitation process must meet at least two physical criteria: (1) it must be reversible, and (2) the protein must retain its native structure in the precipitate. Metal‐induced precipitation is known to meet the first criterion 1–3. In contrast to undesirable, irreversible precipitation that occurs as a result of stress‐induced perturbation of protein structure,6 metal‐precipitated proteins can routinely be solubilized by addition of chelators 1–3.…”

Section: Introductionmentioning

confidence: 99%

“…Metal‐induced precipitation is known to meet the first criterion 1–3. In contrast to undesirable, irreversible precipitation that occurs as a result of stress‐induced perturbation of protein structure,6 metal‐precipitated proteins can routinely be solubilized by addition of chelators 1–3. Retention of native structure, the second criterion, is particularly important when metal‐induced precipitation is used to prepare therapeutic protein formulations because it is widely recognized that long‐term stability of proteins requires storage in the native state 6, 7…”

Section: Introductionmentioning

confidence: 99%

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