Methane (CH4) emissions from animals represent a significant contribution to anthropogenically produced radiatively active trace gases. Global and national CH4 budgets currently use predictive models based on emission data from laboratory experiments to estimate the magnitude of the animal source. This paper presents a method for measuring CH4 from animals under undisturbed field conditions and examines the performance of common models used to simulate field conditions. A micrometeorological mass difference technique was developed to measure CH4 production by cattle in pasture and feedlot conditions. Measurements were made continuously under field conditions, semiautomatically for several days, and the technique was virtually nonintrusive. The method permits a relatively large number of cattle to be sampled. Limitations include light winds (less than approximately 2 m/s), rapid wind direction changes, and high-precision CH4 gas concentration measurement. Methane production showed a marked periodicity, with greater emissions during periods of rumination as opposed to grazing. When the cattle were grazed on pasture, they produced .23 kg CH4 x animal(-1) x d(-1), which corresponded to the conversion of 7.7 to 8.4% of gross energy into CH4. When the same cattle were fed a highly digestible, high-grain diet, they produced .07 kg CH4 x animal(-1) x d(-1), corresponding to a conversion of only 1.9 to 2.2% of the feed energy to CH4. These measurements clearly document higher CH4 production (about four times) for cattle receiving low-quality, high-fiber diets than for cattle fed high-grain diets. The mass difference method provides a useful tool for "undisturbed" measurements on the influence of feedstuffs and nutritional management practices on CH4 production from animals and for developing improved management practice for enhanced environmental quality.

Dry, nonpregnant, mature cows (greater than 10 yr) of five breeds (Angus, A; Brahman, B; Hereford, He; Holstein, Ho; and Jersey, J) and their crosses (n = 60) were used in a 428-d experiment to determine maintenance energy requirements and efficiency of energy exchange. Cows were fed individually (via Calan electronic gates) a 70% cottonseed hull diet for four consecutive periods (127, 105, 97 and 99 d) at each of four levels (50, 83, 117 and 150% of each animal's estimated maintenance requirement). Each of four cows/breed group was assigned to one of the four feeding levels each period, with one cow fed each level each period. Body composition was measured initially and following each period in all cows via D2O dilution with a two-pool kinetics model procedure. Average ending live weight and empty body weight, protein and fat were similar to beginning values, indicating that cows began and ended in similar body composition. Dry matter digestibility (DMD) following the last period averaged 54.7%. Average DMD was 53.5, 57.8, 52.0, 55.0 and 51.7% (standard error of mean [SE] = 2.1) for A, B, He, Ho and J; values for He and J were lower (P less than .05) than for B. Diet digestible energy (DE) was similar for all breed types and averaged 62.4% of gross energy (GE). A small decrease in DE with increasing GE intake was noted for all breed types. The daily metabolizable energy requirement for weight equilibrium for A, B, He, Ho and J differed (P less than .01) and was 100, 98, 108, 119 and 152 kcal/kg.75 (SE 4.8), respectively, with an overall mean of 107. The ME for maintenance (MEm) was 91.6, 93.8, 95.3, 115.7 and 140.4 kcal/kg.75 for A, B, He, Ho and J (SE 6.0), respectively, with an overall mean of 101.9. Efficiency of weight change for A, B, He, Ho and J differed (P less than .01) and was 116, 135, 80, 116 and 58 g/Mcal ME intake, respectively, with an overall mean of 96. The respective efficiency of ME use for tissue energy gain or loss was 80.6, 66.8, 66.0, 36.5 and 36.2% for A, B, He, Ho and J, with an average energetic efficiency of 60.1%. In general, maintenance requirements for weight and energy equilibrium were lower in beef breeds and their crosses than in dairy breeds and their crosses. Efficiency of ME use also favored the beef breeds over the dairy breeds.(ABSTRACT TRUNCATED AT 400 WORDS)

Forty-five Angus steers (avg initial wt 330 kg) were individually fed for 112 d to assess the value of supplemental Zn and source on performance and carcass quality. Steers had ad libitum access to a control diet (81 ppm Zn) of 33% whole corn, 33% ground milo, 15% cottonseed hulls and 13% cottonseed meal, or this control diet with 360 mg Zn/d added from either zinc methionine or zinc oxide. Steers were slaughtered on d 114, and carcass composition was determined by specific gravity. Average daily gain and feed efficiency were not affected by dietary treatments. Steers fed zinc methionine had a higher (P less than .05) USDA quality grade than those fed the control and zinc oxide diets. Marbling score was higher (P less than .05) for steers fed zinc methionine than for those fed control and zinc oxide treatments (4.4 vs 4.0 and 4.0, respectively, where 3 = slight, 4 = small, 5 = modest). Steers fed zinc methionine tended to have more (P less than .10) external fat (13 mm) than steers fed the control diet (10 mm); steers supplemented with zinc oxide had intermediate amounts of external fat (11 mm). Steers fed zinc methionine had 10.5 and 12.8% more (P less than .05) kidney, pelvic and heart (KPH) fat than steers fed control or zinc oxide diets, respectively. The effects of zinc methionine on carcass quality grade and marbling score may be due to Zn and (or) methionine. Regardless of the mechanism, the difference represents a potential economic benefit to producers.

Twenty Angus-Hereford and 20 Angus-Holstein cows were individually fed 12.9 or 18.0 Mcal metabolizable energy (ME)/head daily from November 28, 1979 through February 21, 1980. Energy retentions for the winter feeding period were calculated by determining body composition at the initiation and at the termination of the feeding period. After the experimental period, all cows were managed the same through weaning. Maintenance energy requirements (Kcal/d) estimated from linear regressions of energy retentions on ME intakes per unit body weight (BW).75 were 127.6 and 140.3 kcal/BW.75 for Angus-Hereford and Angus-Holstein cows, respectively. Estimates of maintenance energy requirements for thin and fat cows within each breed type indicated that fatter cows of Angus-Hereford breeding had 6.1% lower energy requirements than thinner cows. Opposite trends occurred with Angus-Holstein cows, where fat cows had 2.7% higher maintenance requirements. Estimated maintenance energy requirements were higher (P less than .01) for protein than fat tissue. Maintenance energy requirements of fat was -1.55 kcal ME/kg for Angus-Hereford cows, indicating that for cows of the same lean body mass, cows with more fat have a lower daily energy requirement during winter. Angus-Holstein cows had an estimated maintenance energy requirement of fat of 51.11 kcal ME/kg. Because cattle of Holstein breeding have less subcutaneous fat than cattle of the beef breeds, and less subcutaneous fat would provide less insulation, the estimated maintenance requirements of fat in Angus-Holstein cows may be an estimate of the true maintenance requirement of fat. Estimates of the partial efficiency of ME use for tissue gain and the ME sparing effect of body tissue loss were 78.8% and .70, respectively, for Angus-Hereford cows and 53.8% and .46, respectively, for Angus-Holstein cows. Regression of retained energy on cow BW.75, body fat and body protein calculated for Angus-Hereford and Angus-Holstein cows from within energy level indicated that BW.75 accounted for less variation in retained energy than weight of empty body fat or protein. Multiple regressions that contained all three variables accounted for 75% and 32% of the variation for Angus-Hereford and Angus-Holstein cows, respectively. Subsequent performance of the cow and calf was not affected by winter energy levels fed, body composition of cows before calving or body energy changes of cows during the winter.

The objectives of this study were to determine growth rates, feed intakes, feed efficiencies, and chemical composition of mice from three transgenic lines in 10-d periods from weaning to near maturity. Lines M4, M11, and G119K express bovine somatotropin (bST) mutations E117L, L121P-E126G, and G119K and display phenotypes of large, near normal, and small body size, respectively. M4 mice were 28% larger at 28 d and 84% larger at 68 d than non-transgenic control (NTC) mice. M11 mice were the same size at 28 d as NTC but were 25% larger at 68 d. G119K mice were 34% and 25% smaller than NTC at 28 and 68 d, respectively. Growth rates of G119K mice and NTC were similar, whereas growth rates of M11 and M4 mice were increased (P < .05). Feed intakes of M4 and M11 mice were greater than those of NTC mice (P < .05), whereas feed intakes of G119K mice were lower than those of NTC mice (P < .05). Feed efficiency (gain/feed) was improved in M4 and M11 mice (P < .05) and not altered in G119K mice compared to that of NTC mice (P > .05). Chemical composition was also altered by expression of bST analogs in transgenic mice. G119K and M4 mice had increased body fat percentages and decreased body protein percentages in comparison to M11 and NTC mice (P < .05).(ABSTRACT TRUNCATED AT 250 WORDS)

No abstract

No abstract

No abstract