Sanquisorba officinalis has been used internally for the treatment of intestinal infections and duodenal ulcers, as well as hemorrhoids, phlebitis and varicose veins and female disorders, and topically to heal wounds, burns, and ulcers. In our study, the antioxidant and antimicrobial activities, as well as quantitative analysis of polyphenols (phenolic acids, flavonoids and total polyphenols) in methanol and aqueous extracts from S. officinalis herbs are presented. A correlation between the antioxidant activity and composition of tested extracts indicates that flavonoids are the major compounds causing scavenging of free radicals. Higher content of flavonoids was found in the methanol extract, while the content of total phenolics was higher in the aqueous extract. Both extracts from S. officinalis herbs showed antioxidant activity and high antimicrobial activity in a wide spectrum of test strains.

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A protocol for C. japonica micropropagation with a confirmation of genome size stability of the in vitro-propagated plantlets was developed. The highest number of shoots multiplied in vitro was obtained on Murashige & Skoog medium (MS) with 1.0 mg L−1 N6-benzyladenine plus 1.0 mg L−1 indole-3-acetic acid. The highest number of roots was observed for the shoots on MS with 15 g L−1 sucrose plus 1.0 mg L−1 indole-3-acetic acid. The acclimatization rate was significantly high. The qualitative HPLC analyses confirmed the presence of phenolic acids and flavonoids in the extracts. The extracts from both shoot cultures and the leaves from field-grown plants revealed antioxidant activity and they exhibited moderate antimicrobial activity. The conducted research confirmed the regeneration potential of genetically-stable plants of C. japonica under in vitro conditions, the ability of the plantlets to produce polyphenols as those present in field-grown plants, as well as their antioxidant potential.

The chemical composition of Succisa pratensis is not well known. The existing data indicate a substantial content of flavonoids, which include luteolin and apigenin 7-glucosides. The aim of this study was to elaborate the isolation protocol of these flavonoids from flowers and leaves of S. pratensis, to carry out their characterization, as well as evaluate the effect of S. pratensis extracts on activation of transcription factor NF-κB and α-amylase activity. The extraction protocol applied in this study allowed isolation and characterization of flavonoid fraction of S. pratensis. Their identity was confirmed by NMR spectra analysis, UV spectroscopy and electrospray ionization-tandem MS evaluation. Treatment of pancreatic α-amylase with S. pratensis extract inhibited this enzyme’s activity to an extent comparable to that of isolated luteolin and apigenin 7-glucosides. Incubation of HepG2 cells for 24 h with S. pratensis extracts or isolated flavonoids resulted in moderate reduction in NF-κB transcription factor activation evaluated in terms of translocation of its active subunits from cytosol into nucleus and subsequently diminished expression of the COX-2 gene. Expression of NF-κB was also reduced. The most significantly diminished NF-κB activation and expression, as well as COX-2 expression, was found to result from treatment with isolated flavonoids and ethyl acetate extract of S. pratensis leaves. These results indicate that S. pratensis flavonoids may modulate the metabolic and signaling pathways whose deregulation is related to pathogenesis of liver cancer. Further studies are required to confirm these observations and assess the chemopreventive and/or therapeutic potential of the S. pratensis herb.

Three species of the genus Trollius (Ranunculaceae) are traditionally used to treat upper respiratory tract infections, pharyngitis, tonsillitis, bronchitis, cold with fever, acute tympanitis, aphthae, mouth sore, hemorrhage and pain of gums, acute lymphangitis and acute periostitis. However, only a few studies support its traditional use. These are studies of the biological activity of extracts and/or compounds of selected species of Trollius, but there are no clinical studies proving the effectiveness or possible toxic effects. Until now, the following activity of extracts and/or compounds from certain species of Trollius used in traditional medicine has been proven: antiviral, antibacterial, antiinflammatory and antioxidant. The review showed that flavonoids, mainly C-glycosides, were characteristic of the species Trollius. Furthermore, other main groups of compounds are carotenoids, organic acids, terpenes, alkaloids, sterols, lactones and carbohydrates. The essential oil mainly contains compounds from the group of benzenoids, nitrogen-containing compounds, monoterpenoids and sesquiterpenoids, irregular terpenes and macrocyclic epoxide.

A new flavonoid compound, 40 -O-a-rhamnopyranosyl (1 ? 2)-b-xylopyranoside of 7-O-methylapigenin (1) was isolated from the leaves of Trollius europaeus. The structure of this compound was elucidated by spectroscopic methods (UV, 1D NMR, 2D NMR and MS). Compound (1) was analyzed for antioxidant activity in DPPH assay.

The results suggest great antibacterial activity of the oils and high antioxidant activity of the methanol extract and may justify the application in treating infections.

The influence of stress factors on a plant may lead to worse functioning of the plant and the loss of its crop. The effect of Aulacorthum solani feeding on Trollius europaeus with regard to active compounds in the leaves was investigated in the study. The antioxidant activity of the leaves, the material on which the insects fed, was compared with that of the material that was not infested by the aphids. Stress level was evaluated on the basis of such physiological parameters as chlorophyll fluorescence and photosynthesis activity. An increase of 34.5% in the content of polyphenolic compounds, as compared to control, was demonstrated in the material exposed to the biotic stress caused by aphids. The content of phenolic acids was 28% higher while that of flavonoids rose by 25%. The increase in polyphenolic compounds augmented the antioxidant activity of the material.