Micropropagation of Chaenomeles japonica: A Step towards Production of Polyphenol-rich Extracts Showing Antioxidant and Antimicrobial Activities

Kikowska, Małgorzata; Włodarczyk, Agata; Rewers, Monika; Śliwińska, Elwira; Studzińska-Sroka, Elżbieta; Witkowska-Banaszczak, Ewa; Stochmal, Anna; Żuchowski, Jerzy; Długaszewska, Jolanta; Thiem, Barbara

doi:10.3390/molecules24071314

Cited by 18 publications

(16 citation statements)

References 32 publications

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“…Similar amounts (from 42.90% to 50.90%) of hydroxycinnamic acids in quince leaves were found in a recent study of Chojnacka and co-authors [ 13 ]. The predominant compound of this group in all cultivars was chlorogenic acid, and this is in agreement with other studies [ 13 , 19 ]. The amount of flavonols in all cultivars was around 30% of total phenols.…”

Section: Discussionsupporting

confidence: 93%

Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

Žvikas

Urbanavičiūtė

Bernotienė

et al. 2020

Foods

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Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g (‘Rondo’) to 17,048 µg/g DW (‘Darius’). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs.

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Section: Discussionsupporting

confidence: 93%

Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

Žvikas

Urbanavičiūtė

Bernotienė

et al. 2020

Foods

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“…This confirmed the genome size stability of micropropagated material, which is in agreement with the results obtained by ISSR, revealing no somaclonal variation in plantlets. Similar stability has been found for numerous species produced in vitro, including trees and shrubs, e.g., oak, eucalyptus, banana, olive, and Japanese quince [54][55][56][57][58].…”

Section: Flow Cytometrysupporting

confidence: 75%

Genetic Stability of the Endangered Species Salix lapponum L. Regenerated In Vitro during the Reintroduction Process

Parzymies

Pogorzelec

Głębocka

et al. 2020

Biology

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Salix lapponum L. is a boreal relict species, threatened with extinction in Poland. An 80% decrease in the number of its stands was confirmed in the last half-century, so that to prevent the loss of downy willow, attempts were made to reintroduce this species in natural habitats. Micropropagation was chosen as a first stage of its active conservation. S. lapponum shoots were collected and disinfected with NaOCl, AgNO3, or HgCl2 or with a two-step disinfection with NaOCl and then placed on MS medium with BA 1 mg·dm−3 and IBA 0.1 mg·dm−3. Regenerated shoots were cultivated with addition of BA, KIN, or 2iP, alone or in combination with auxins, to find the highest multiplication rate. Inter-simple sequence repeat (ISSR) analysis and flow cytometric analyses were conducted on in vitro regenerated plants to check their genetic stability. Disinfection was quite difficult and the use of HgCl2 was the most efficient. The highest multiplication rate was obtained in presence of KIN at 0.5 mg·dm−3 + IAA at 0.5 mg·dm−3. The analysis confirmed the genome size stability, which is in agreement with the results obtained by ISSR, revealing no somaclonal variation in plantlets and therefore allowing the use of the obtained plants for reintroduction.

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“…In addition, rutin showed the ability to enhance the antibacterial activity of other phenols and antibiotics [51,52]. JQ fruit extracts had a stronger inhibitory effect against E. coli ATCC 25922 and P. aeruginosa ATCC 27853 than S. aureus ATCC 25923 [20]. The results of our study showed that only 'Rasa' extracts have similar activity, but 'Darius' and 'Rondo' extracts had the opposite effect, stronger inhibits S. aureus (ATCC 25923).…”

Section: Propertiesmentioning

confidence: 60%

“…Numerous studies have shown that the phenolic compounds are promising biologically active compounds that may act as a new type of antimicrobial agent [17][18][19]. Kikowska and co-authors demonstrated the antibacterial activity of JQ leaf and fruit extracts against four bacteria strains Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and one yeast strain Candida albicans (ATCC 10231) [20]. The antibacterial activity of other species such as Chaenomeles speciosa essential oil against 10 microorganisms has been studied [21].…”

Section: Introductionmentioning

confidence: 99%

Japanese Quince (Chaenomeles japonica) as a Potential Source of Phenols: Optimization of the Extraction Parameters and Assessment of Antiradical and Antimicrobial Activities

Urbanavičiūtė

Liaudanskas

Bobinas

et al. 2020

Foods

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The value of fruits is determined by the quantity and variety of biologically active compounds they contain, and their benefits on human health. This work presents the first study of the biochemical composition and antibacterial activity of the new Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ fruits. The total phenolic content (TPC) was determined using the Folin–Ciocalteu method and each compound was identified by HPLC High Performance Liquid Chromatography. The antimicrobial activity against three Gram-positive and three Gram-negative bacteria, and one yeast strain, was evaluated by the agar well diffusion method using three different concentrations. The free radical scavenging activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid) methods and ranged from 99.1 to 115.9 μmolTE/100 g, and from 372 to 682 μmolTE/100 g, respectively. TPC ranged from 3906 to 4550 mgGAE/100 g, and five compounds, isoquercitrin, rutin, (+)-catechin, (–)-epicatechin, and chlorogenic acid were identified. All JQ extracts possessed antimicrobial activity against Gram-positive and Gram-negative bacteria, and Enterococcus faecalis (ATCC 29212) was the most sensitive strain. These results indicate that JQ fruits are a significant source of bio-compounds, which can enrich the diet with strong antioxidants, and they are very promising as a substitute for chemical preservatives in the food and cosmetic industry.

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Micropropagation of Chaenomeles japonica: A Step towards Production of Polyphenol-rich Extracts Showing Antioxidant and Antimicrobial Activities

Cited by 18 publications

References 32 publications

Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

Genetic Stability of the Endangered Species Salix lapponum L. Regenerated In Vitro during the Reintroduction Process

Japanese Quince (Chaenomeles japonica) as a Potential Source of Phenols: Optimization of the Extraction Parameters and Assessment of Antiradical and Antimicrobial Activities

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