Purpose Psychoactive compounds that contain a phenylethylamine structure (such as amphetamine-type stimulants and synthetic cathinones) are one of the major classes of stimulants on the recreational drug market. Approximately 670 new psychoactive substances (NPS) are monitored only in Europe; however, new psychoactive compounds are being developed for illicit trade each year. In this context, the development of new analytical procedures for the determination of such compounds in biological specimens for forensic toxicology is of great importance. Methods Gas chromatography-tandem mass spectrometry (GC-MS/MS) technique was applied for analysis of amphetamines and synthetic cathinones. The volumes of 200 µL of each whole blood sample and 1 mL of liquid-liquid extraction solvent were used for extraction, followed by pentafluoropropionyl derivatization. Results A high-throughput, robust, rapid, and sensitive procedure involving a simple liquid-liquid extraction for the simultaneous determination of 45 amphetamine-type stimulants and synthetic cathinones in whole blood was developed. The assay was validated based on its recovery (83.2-106%), interday accuracy (89.0-108%), and interday precision (≤ 8.1%). In view of the low limits of detection (ranged between 0.02 and 0.72 ng/mL) and limits of quantification (1 and 2.5 ng/mL), the developed method can serve as a less expensive and more ecologically friendly alternative to the liquid chromatography-tandem mass spectrometric methods. Conclusions To the best of our knowledge, this is the first work presenting a GC-MS/MS method for the determination of NPS in blood samples. The presented procedure was applied to authentic samples from forensic cases, demonstrating its utility in the quantification of a wide number of psychoactive substances in routine toxicological analyses. The developed procedure can also be easily expanded to additional compounds. Keywords Amphetamine-type stimulants (ATSs) • Synthetic cathinones • Whole blood • GC-MS/MS Prof. Jacek Namieśnik passed away on 14 April 2019. He will always remain in our memory.
PurposeThe 4-chloromethcathinone (4-CMC) is a synthetic derivative of cathinone and belongs to new psychoactive substances. Neither data on the effects of 4-CMC on the human body, nor on nontoxic, toxic and lethal concentrations in biological materials have been published in the literature. This paper describes the results of an analysis of the blood concentrations of 4-CMC determined in 15 forensic cases related to nonfatal intoxication including driving under the influence, and fatalities including overdoses, suicide and traffic accidents.MethodsA new method for the quantification of 4-CMC using gas chromatography–mass spectrometry (GC–MS) was developed. The symptoms of 4-CMC use were also studied based on an analysis of the documents prepared during the collection of samples or at autopsies.ResultsThe limits of detection and quantification of the method for blood samples were 0.3 and 1 ng/mL, respectively. The calibration curve was linear in the studied concentration range (1–500 ng/mL) with the correlation coefficient at 0.9979. The extraction recoveries varied in the range of 94.3–98.8%. The accuracy and precision were acceptable. The determined concentrations in nonfatal cases ranged from 1.3 to 75.3 ng/mL, and in fatalities from 56.2 to 1870 ng/mL.ConclusionsOur study can assist in the recognition of the possible effects caused by 4-CMC and can be helpful during the preparation of forensic toxicological opinions for courts of law. The validation parameters indicate the sensitivity and accuracy of the method. This is the first work presenting a validated method for the determination of 4-CMC in blood samples by GC–MS.
Left handers have long held the edge over right handers in one-on-one interactive combat sports. Particularly in fencing, top rankings show a relatively strong overrepresentation of left handers over right handers. Whether this can be attributed to perceptual strategies used by fencers in their bouts remains to be established. This study aims to verify whether right-handed fencers assess their opponents’ behaviour based on different perceptual strategies when fencing a left vs. right hander. Twelve top-level (i.e., Olympic fencers, Junior World Team Fencing Champions, and top Polish senior foil fencers) right-handed female foil fencers (aged 16–30 years) took part in the study. They performed a total of 40 actions: 10 repetitions of offensive actions (attack) and 10 repetitions of defensive actions (defence), each type of action performed under 2 conditions (right- vs. left-handed opponent). While the participants were fencing, their eye movements were being recorded with a remote eye-tracker (SMI ETG 2.0). Both in their offensive and defensive actions, the fencers produced more fixations to the armed hand and spent more time observing the armed hand in duels with a left-handed (vs. right-handed) opponent. In defence, it was also the guard that attracted more fixations and gained a longer observation time in bouts with a left hander. In duels with a right-handed opponent, a higher number of fixations in attack and in defence, and longer observation times in defence were found for the upper torso. The results may point to different perceptual strategies employed in bouts with left- vs. right-handed individuals. The findings from this study may help to promote the implementation of specialized perceptual training programmes in foil fencing.
We examine cross‐linguistic influence in the processing of motion sentences by L2 users from an embodied cognition perspective. The experiment employs a priming paradigm to test two hypotheses based on previous action and motion research in cognitive psychology. The first hypothesis maintains that conceptual representations of motion are embodied by sensory and motor systems (Glenberg & Kaschak, 2002; Zwaan & Taylor, 2006) and predicts that linguistic descriptions of motion will be susceptible to conceptual priming effects. The second hypothesis assumes that the processing of fictive motion sentences, such as The road runs through the forest (cf. Talmy, 2000a), involves additional mental simulation of motion (Matlock, 2004b; Richardson & Matlock, 2007) and predicts that fictive motion will be processed more slowly than real motion. The participants were groups of Polish and English monolinguals and Polish L2 users of English, who were students in an English‐medium university program. Sentence meaningfulness judgment and response time data were collected from the monolinguals and the L2 users tested in both languages. Sentence meaningfulness judgments were examined to see how the participants represent motion in language and response time data were analyzed for evidence of conceptual processing. The results showed expected differences in response times to various sentence types in all groups, but the priming effect was not observed in Polish monolinguals and only selectively present in the L2 users responding in both languages. The results of our experiment indicate that fictive motion processing is both embodied in physical experience of motion and modified by language.
Effective information behaviour is crucial in all translation competence models but our understanding of how information skills develop and how translators interact with information found in online resources is still limited. In this article we focus on information behaviour (needs and use) of bidirectional translators who frequently translate into their native (L1) and their non-native language (L2). The theoretical underpinnings come from information studies: (1) information is needed when cognitive uncertainty arises and -when found -it allows the translator to make an informed decision; (2) translators are driven by economy of effort and will minimise the cost of searching for information. The empirical evidence comes from a study of 30 professional bidirectional translators who translated two texts into their native language of low diffusion (Polish) and into their non-native major language (English). A close analysis of their information behaviour included data obtained by keylogging, eye-tracking and screen recording, and showed that using online resources adds more cognitive effort when translators work into their L2. We use the results to draft a model of information behaviour which shows how the use of online resources is affected by the translation direction.
The paper presents a unique case of a complex suicide committed by a young man, mostly probably triggered by a disappointment in love. The uniqueness of the suicide lies in the fact that the victim inflicted several deep stab wounds on himself, in the chest and abdomen, while standing partly submerged in the sea and, having done so, he dropped and disappeared in the water. The postmortem examination showed, apart from deep wounds in the trunk, characteristics of drowning that manifested itself in the form of aqueous emphysema of the lungs. Suicide was clearly determined on the basis of the circumstances preceding death, the location, and arrangement of the trunk wounds and the testimony given by a witness of the incident. The circumstances preceding the suicidal act clearly suggest an underlying undiagnosed mental disorder.
An effective visual perception strategy helps a fencer quickly react to an opponent’s actions. This study aimed to examine and compare visual perception strategies used by high-performance foil fencers (experts) and beginners. In an eye tracking experiment, we analysed to which areas beginning and expert fencers paid attention during duels. Novices paid attention to all examined areas of interest comprising the guard, foil (blade and tip), armed hand, lower torso, and upper torso of their opponents. Experts, however, paid significantly less attention to the foil, picking up information from other areas, mainly the upper torso and the armed hand. These results indicate that expert fencers indeed engage different visual perception strategies than beginners. The present findings highlight the fact that beginner fencers should be taught already in the early stages of their careers how to pick up information from various body areas of their opponents.
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