In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous layer with micron-sized defects on the nanotitanium surface. The present work continues an earlier study by our group, wherein the effect of osteoblast-like cell adhesion acceleration was found. In the present paper, we give the results of more detailed evaluation of coating efficiency. Specifically, cytological analysis was performed that included the study of the marker levels of osteoblast-like cell differentiation. We found a significant increase in the activity of alkaline phosphatase at the initial incubation stage. This is very important for implantation, since such an effect assists the decrease in the induction time of implant engraftment. Moreover, osteopontin expression remains high for long expositions. This indicates a prolonged osteogenic effect in the coating. The results suggest the acceleration of the pre-implant area mineralization and, correspondingly, the potential use of the developed coatings for bone implantation.

Modern materials science, both in terms of functional and structural materials, is actively developing towards the creation of structures with a given ordering. A wide range of methods involves ordering the structure according to a template shape. Template synthesis is one of the mots wide-spread approaches. Most often, the template synthesis method is implemented under conditions of limiting the growth of the phase due to the geometry of the template. In the present work, a template electrochemical method is considered for calcium hydroxyapatite (HAp) coating synthesis, based on the replication of the planar template texture during deposition. In this case, the template is an array of silver microparticles immobilized on an electrically conductive substrate, separated by an insulator layer. The developed approach is similar to the mask metallization widely used in planar technology. In this work, the possibility of the template pulsed electrodeposition of ceramics rather than metal is shown using HAp as an example. This approach is interesting for materials science, in particular, for obtaining micro-ordered hydroxyapatite structures—a crystallochemical analogue of the inorganic bone tissue component—on the surface of bone implants, which can be implemented to improve their biomedical characteristics. As a result of our study, we experimentally determined the conditions for obtaining the composite coating TiO2/Ag/Ca10(PO4)6(OH)2 with controlled phase structure, topology and localization of components on the surface, which was confirmed by Scanning Electron Microscopy, Energy Dispersive Spectroscopy, and X-ray Diffraction (SEM, EDS and XRD). The absence of cytotoxicity for the osteoblast-like cells of the developed coating was revealed by cytological tests.

No abstract

This work is devoted to the development of non-lithographic template methods of synthesis. These methods have a significant advantage in terms of structure formation: there is no need to design and produce masks, which greatly simplifies the process, and more of them can work with nonplanar substrates. The purpose of this study was to reveal the conditions for the synthesis of titanium dioxide xerogel films of different topologies as well as to develop a technique for non-lithographic template electrochemical synthesis of micron metal particles arrays and to study the structure of the resulting coatings. The films were deposited on the surface of substrates via dip coating. Specific topology of the films was achieved by template sol-gel synthesis. Their structures were analyzed by SEM and XRD. Template synthesis of metal micro particles were realized by pulsed electrochemical deposition of metals into the perforations of xerogel films. Obtained materials were analyzed by SEM and XRD; the element distribution on the surface was determined by the EDS detector of SEM. Based on the analysis results, we suggest the mechanisms of formation of the xerogel topology and proved the efficiency of pulsed electrodeposition for template synthesis of micron particles arrays.

No abstract

No abstract

No abstract

No abstract