Bioactive Coating with Two-Layer Hierarchy of Relief Obtained by Sol-Gel Method with Shock Drying and Osteoblast Response of Its Structure

Abstract

In this work, we analyze the efficiency of the modification of the implant surface. This modification was reached by the formation of a two-level relief hierarchy by means of a sol-gel approach that included dip coating with subsequent shock drying. Using this method, we fabricated a nanoporous layer with micron-sized defects on the nanotitanium surface. The present work continues an earlier study by our group, wherein the effect of osteoblast-like cell adhesion acceleration was found. In the present paper, we give the results of more detailed evaluation of coating efficiency. Specifically, cytological analysis was performed that included the study of the marker levels of osteoblast-like cell differentiation. We found a significant increase in the activity of alkaline phosphatase at the initial incubation stage. This is very important for implantation, since such an effect assists the decrease in the induction time of implant engraftment. Moreover, osteopontin expression remains high for long expositions. This indicates a prolonged osteogenic effect in the coating. The results suggest the acceleration of the pre-implant area mineralization and, correspondingly, the potential use of the developed coatings for bone implantation.

Three-component working electrode micron-sized Ag particles/tiO2 layer/Ti: template electrochemical synthesis and potential use as electrochemical sensor for glutathione detection

“…The extraction speed was 100 mm min −1 . The deposited film was heat treated on the hot plate at 400°C, which results, as previously shown [11], in partial cracking. After that, film was extracted with boiling deionized water and air dried at 200°C.…”

Role of implants surface modification in osseointegration: A systematic review

“…Adams et al produced a vancomycin‐containing sol–gel film on Ti alloy, which exhibited predictable release kinetics and successfully treated the bacterial infection in a rat osteomyelitis model (Adams et al, ). In addition, the sol–gel method combined with different coating processes can create bioactive coatings with a multiple‐layer hierarchy, which corresponds with the bone structure (Zemtsova et al, ).…”

Features of Fabrication of Titanium Dioxide Based Coatings for Non-Lithographic Template Electrochemical Synthesis of Micron Metal Particle Arrays

“…For example, a macroporous ordered polymer coating can be obtained by template synthesis using a spin-coated colloidal crystal [ 27 ], and an array of silver nanorods oriented normally to the substrate can be obtained by template electrochemical synthesis using anodic films [ 28 ]. There are many such coatings and many of them are useful for practical applications in various fields, for example, in optics [ 29 , 30 , 31 ], microelectromechanical systems [ 32 , 33 , 34 ], sensors [ 35 , 36 , 37 ], and medicine [ 38 , 39 , 40 ].…”