Three-component working electrode micron-sized Ag particles/tiO₂ layer/Ti: template electrochemical synthesis and potential use as electrochemical sensor for glutathione detection

Abstract

In the present work the possibility is considered of a chemical sensor synthesis for quantitative glutathione (GSH) determination. Sensor is based on a composite working electrode containing an array of micron-sized Ag particles immobilized on a conductive substrate (Ti) coated by dielectric TiO 2 film. To determine GSH in biological fluids, particularly, in saliva, electrochemical silver-based sensors can be used, since such sensors contain -SH group. With the use of cyclic voltammetry (CV) with a composite working electrode containing an Ag microparticles array, the threshold of quantitative GSH determination is reduced to nM level. Since other modern analogues are inferior at least one order of magnitude in the limit of quantitative GSH detection, we assume that the proposed sensor may be of great interest for clinical diagnosis.

A Substituted‐Rhodamine‐Based Reversible Fluorescent Probe for In Vivo Quantification of Glutathione

“…[2] Thus, the dynamic of GSH in tissues or living animals is essential in unveiling the related physiological processes and pathogenesis of the diseases, including disease initiation, progression, and response to treatment in living biosystems. [3] Various methods, such as chromatography, [4] mass spectrometry, [5] Raman spectroscopy, [6] electrochemical methods, [7] magnetic resonance imaging, [8] colorimetry, [9] et al, have been employed in the determination of GSH in biological systems. However, most of these established methods are not suitable for rapid and convenient detection of GSH, peculiarly in living cells and tissues, owing to the required pre-treatment with the complicated operation and prolonged analysis.…”

“…Various methods, such as chromatography, [4] mass spectrometry, [5] Raman spectroscopy, [6] electrochemical methods, [7] magnetic resonance imaging, [8] colorimetry, [9] et al., have been employed in the determination of GSH in biological systems. However, most of these established methods are not suitable for rapid and convenient detection of GSH, peculiarly in living cells and tissues, owing to the required pre‐treatment with the complicated operation and prolonged analysis.…”

Creation of a Composite Bioactive Coating with Antibacterial Effect Promising for Bone Implantation

“…Composite coatings based on silver and HAp have good biocompatibility and antibacterial properties [ 26 , 27 , 28 ]. However, the published methods do not allow for the obtaining of the desired surface with a complex micro- and multi-level structure [ 29 ].…”

Template Electrochemical Synthesis of Hydroxyapatite on a Titania–Silver Composite Surface for Potential Use in Implantology

“…In this work, we take pre-synthesized TiO 2 /Ag matrix [42] with a complicated microlevel morphology as the template. We develop the technique of electrochemical HAp synthesis on the TiO 2 /Ag template surface in order to create a composite TiO 2 /Ag/HAp film with a controlled structure and composition: pulsed electrochemical deposition, in contrast to DC deposition, achieves the replication of the template structure by depositing hydroxyapatite.…”