Echinaceae is introduced medicinal plant as immunomodulator. The high demand of echinaceae as raw material for immunomodulatory herbal medicine by the industries, requires the increase in quantity and quality of cultivated echinaceae. Thus, the use of fertilizer is necessary. However, to ensure the safety of raw materials for herbal medicine industry, the use of organic fertilizer becomes one of the important components. Organic fertilizer used can be in the form of solid fertilizer such as dung manure, compost and green manure. Various plants can be utilized as a source of green manure such as Tithonia diversifolia, because of its high N content. The aim of this research was to evaluate the effect of the dosage of dung manure and green manure from <em>T. diversifolia</em> on echinaceae biomass and echinacoside contents. The research was arranged in Factorial Randomized Block Design with two treatments, three dosages of dung manure (P) 0; 2.5; 5 ton.ha<sup>-1</sup>, and three dosages of green manure from <em>T. diversifolia</em> (T) 0; 10 and 20 ton.ha<sup>-1</sup>. The treatment combinations were repeated three times. The results showed that there was no interaction between <em>T. diversifolia </em>green manure and dung manure usage. The use of <em>T. diversifolia</em> fertilizer alone was not evidently increased the fresh weight of biomass at 20 ton.ha<sup>-1</sup>. The use of <em>T. diversifolia</em> and dung manure showed no significant effect on echinacoside content.
<em>Gotu kola is one of the medicinal plants that has begun to be cultivated by the Indonesian people. It’s cultivation needs to follow the GAP to produce the qualified yield, including the use of fertilizers. Biofertilizers have been widely developed to reduce adverse effects of chemical fertilizers to the environment, thus an organic cultivation method can be an alternative. The aim of this study was to determine the effect of biofertilizer on growth and yield of gotu kola. This research was an experimental study with a completely randomized design (CRD) using 8</em> <em>planting media treatments consisting of (a) Soil + manure + dolomite; (b) Soil manure + 1 g biofertilizer; (c) Soil + dolomite + 1g biofertilizer; (d) Soil + manure + 3 biofertilizer; (e) Soil + dolomite + 3 biofertilizer; (f) Soil + manure + 5 g biofertilizer; (g) Soil + dolomite + 5 g biofertilizer; and (h) Soil + 3 g</em> <em>biofertilizer. Parameters observed were leaf area, stem length, number of leaves, number of tillers, fresh weight, dry weight and asiaticoside content. The results showed that combination treatment of biofertilizer (1 g) with dolomite (30 g) gives better effect in increasing the number of tillers and number of leaves, while the combination of biofertilizer (5 g) and dolomite (30 g) is the best for asiaticoside levels in gotu cola.</em>
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