The objective of this study was to evaluate the interaction between controlled atmosphere (CA), CA+1-methylcyclopropene (1-MCP) and dynamic controlled atmosphere monitored by respiratory quotient (DCA-RQ) with three fruit maturity stages at harvest (early harvest date, optimal harvest date and late harvest date) on 'Galaxy' apple metabolism and quality after harvest and 9months storage plus 7days of shelf life at 20°C. Fruit stored under dynamic controlled atmosphere monitored by respiratory quotient 1.3 (DCA-RQ 1.3) showed lower ethylene production, respiration rate, mealiness and higher flesh firmness in comparison to CA stored fruit, but did not differ from those treated with 1-MCP. The dynamic controlled atmosphere monitored by respiratory quotient 1.5 (DCA-RQ 1.5) increased the acetaldehyde, ethanol and ethyl acetate concentration, regardless of the fruit maturity at harvest. The storage of 'Galaxy' apple under DCA-RQ 1.3 is efficient in keeping quality regardless of the maturity stage at harvest.

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Growth regulators are used in the production of apples worldwide, especially to extend the harvest period and maintain postharvest quality. This study aimed to investigate the effects of applying aminoethoxyvinylglycine (AVG) in isolation as well as in combination with other growth regulators and postharvest techniques on the harvest quality and storage potential of 'Brookfield' apples (Malus domestica), a 'Gala' strain. Fruit receiving AVG only had the highest starch content and the highest titratable acidity at harvest. After 8 months of storage, the AVG + 1-MCP (1-methylcyclopropene) and AVG + ABS (ethylene absorption) conserved higher flesh firmness than to all the other treatments. Naphthaleneacetic acid (NAA) application induced ACC oxidase enzyme activity at harvest, but not after storage. AVG application, with or without the aid of another technique, did not decrease the red skin color of 'Brookfield' apples. Low mealiness and a high healthy fruit percentage was obtained when the fruits were submitted to pre-harvest AVG application combined with NAA, 1-MCP and ABS. Internal carbon dioxide had an inverse correlation with the quantity of healthy fruit and was directly correlated with mealiness.Keywords: Malus domestica, postharvest quality, skin color, ethephon, naphthaleneacetic acid IntroductionThe application of growth regulators is necessary either to advance or to delay the fruit harvest in the field, due to the short period that fruit remains naturally at the correct maturation stage. Besides the short harvest period, another problem is that the harvest is carried out manually and requires a lot of time and manual labor. Thus, growth regulators are frequently used in the orchards.The main growth regulator applied in the field is aminoethoxyvinylglycine (AVG) which is used to delay the harvest in order to avoid pre-harvest fruit drop (McFadyen et al., 2012;Yildiz et al., 2012). AVG blinds the active site of ACC (1-aminocyclopropane-1-carboxylate) synthase enzyme and inhibits ethylene production and maturation events initiated by this plant hormone (Yu and Yang, 1979;Huai et al., 2001). Despite the benefits promoted by AVG application, it decreases the red skin color of 'Gala' apples (Malus domestica) (Steffens et al., 2006). However, this reduction of red skin color has not yet been proven in 'Gala' mutants.Another growth regulator used extensively is ethephon, mainly to advance the fruit harvest. Its application allows for an early harvest of the fruit, and also increases the red color of fruit skin Steffens et al., 2006;Ban et al., 2007). However, ethephon application may decrease organoleptic quality and decrease the storage potential of fruit after harvest, especially in terms of declines in flesh firmness (Steffens et al., 2006;Ban et al., 2007). As well as the application of ethephon, naphthaleneacetic acid (NAA) is also used during apple production. Its application reduces pre-harvest fruit drop in apples (Yuan and Carbaugh, 2007; Unrath et al., 2009), oranges (Zur and Goren, 1977) ...

-The aim of this work was to evaluate the effect of ethanol and two nitric oxide dose applications on the maintenance of the post-storage quality of 'Galaxy' apple during storage under controlled atmosphere (CA) nitric oxide showed lower ethylene production, respiration rate and ACC oxidase ( (1-aminocyclopropane-1-carboxylic acid) oxidase enzyme activity. Apples treated with 20 uL L -1 nitric oxide showed higher ethylene production, respiration rate, internal ethylene concentration CO 2 and ethanol concentration. Ethanol and nitric oxide application before storage have no benefits in maintaining fruit quality after storage under CA due to lower flesh firmness, higher mealiness incidence, flesh breakdown and decay incidence. Index terms: Ethylene, respiration rate, fermentation compounds, Malus domestica. Os frutos foram mantidos em AC durante oito meses e sete dias a 20º C. Frutos tratados com etanol apresentaram maior produção de etileno, menor firmeza, elevada degenerescência, polpa farinácea e produção de acetaldeído. Já, os frutos tratados com 40 µL L -1 de óxido nítrico apresentaram menor produção de etileno, taxa respiratória e atividade da enzima ACC (1-aminociclopropano-1-carboxílico) oxidase. Maçãs tradadas com 20 µL L -1 de óxido nítrico apresentaram aumento na produção de etileno e respiração, etileno e CO 2 interno, e produzem mais etanol. A aplicação de etanol e óxido nítrico não apresenta benefícios na manutenção da qualidade dos frutos após o armazenamento em AC, devido à menor firmeza de polpa, maior ocorrência de polpa farinácea, degenerescência de polpa e incidência de podridões nos frutos. Termos para indexação: Etileno, taxa respiratória, compostos da fermentação, Malus domestica. ETANOL E ÓXIDO NÍTRICO NA MANUTENÇÃO DA QUALIDADE DE MAÇÃS 'GALAXY' ARMAZENADAS EM

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