ABSTRACT:The present study examined the results of using hyaluronic acid with autogenetic cancellous grafts in the treatment of experimentally induced osteochondral defects in the stifle joints of dogs. In this study, 10 mature dogs of different breeds, weights and of both sexes were used. General anesthesia and usual operation procedures were followed. A 10 mm deep defect was created on the femoral sulcus of the trochlea with a drill tip of 8 mm in diameter. The defects in the right and left legs were filled with autogenic cancellous grafts taken from the metaphysial region of the tibia. The left legs constituted the experimental group while the right legs served as control group. In the experimental group, 2 mg/kg intraarticular hyaluronic acid was twice administered into the stifle joint, i.e., immediately subsequent to the operation and 1 month afterwards. Parenteral antibiotics were prescribed postoperatively for ten days. Five animals were sacrificed at the third and sixth month after surgery. Macroscopic and microscopic findings obtained from each case were evaluated. On macroscopical examination, trochlear defects were determined to be incompletely filled at the third month in both control and experimental groups. On histopathologic examination, a loose fibrovascular formation in the area where the graft was applied was observed to be present in both control and experimental groups. However, in the experimental group this formation was more superficial, ossification activity was greater and trabeculous bone formation had been initiated. Macroscopical examination carried out in the sixth month determined that in the control group the defect surface did not fill up to the trochlear sulcus level. In the histopathologic examination, in control groups it was found that fibrocartilageous structures were developing in the fibrovascular space even though ossification was incomplete. The macroscopic examination showed that in the experimental group, the defect surface reached the trochlear sulcus level of defects in this month. The histopatologic examination revealed that fibrous tissue comprised a thin layer, under which ossification processes were complete and bone trabeculates fully formed. It was concluded that the usage of autogenic cancellous graft along with hyaluronic acid may be useful in the repair of large osteochondral defects.
This study was undertaken to investigate the effectiveness of polypropylene (PP) mesh coated bovine amniotic membrane (BAM) with 5% polyethylene glycol (PEG) 4000 as adhesion barrier in the repair of experimental 2 × 2 cm of abdominal hernias in rats. Rats (32) were divided into 4 groups. A 2 cm × 2 cm defect was created in the full thickness of abdominal muscle on the anterior abdominal wall. PP mesh was implanted in the abdominal cavity (Gr 1 to 4). BAM covered the abdominal face of the graft (Gr 3 and 4). It was given before the abdominal closure 5 ml of 5% PEG 4000 (Gr 2 and 4) and 5 ml of 0.9% NaCl (Gr 1 and 3). After 21 days following the operations, 32 rats were euthanized. Macroscopic and microscopic evaluations were performed according to the scoring systems. The differences between the groups was evaluated by Mann-Whitney U test and Kruskal Wallis analysis of variance. Highest adhesion percentage was observed in Gr 1 and lowest in Gr 4. Inflammatory reaction was observed in Gr 1 and 2. According to the results of this study, the combined use of BAM and 5% PEG 4000 was helpful to prevent the complications of PP mesh.
Bu çalışmanın materyalini, Fırat Üniversitesi Veteriner Fakültesi Cerrahi Kliniğine başın oksipital bölgesinde kitle ve ayağa kalkamama şikayetiyle getirilen, üç günlük, erkek Holştayn buzağı oluşturdu. Buzağının, genel muayenesinde oksipital bölgede 9x17cm boyutlarında fluktuan bir kitle gözlendi. Başın, laterolateral pozisyonda yapılan radyografik muayenesi sonucunda kitlenin sıvı ile dolu olduğu ve os occipitalis'te, 4 cm çapında açıklığın bulunduğu saptandı. Kitlenin eksizyonu yapıldı. Postoperatif dönemde on gün süre ile parenteral Seftriakson (Unacefin, Fako) uygulandı. Postoperatif dönemde bilgisayarlı tomografi (BT) ile beyin ve diğer dokularda başka bir defektin olup olmadığı araştırıldı. Olgunun BT'sinde başka bir defekte rastlanmadı. Operasyondan bir gün sonra hayvanın ayağa kalkabildiği, sütünü emebildiği ancak yürüyüşünde diskordans gözlendi. Postoperatif onuncu günde dikişleri uzaklaştırıldığında olgunun tüm fonksiyonlarının normal olduğu belirlendi. Olgunun iki yaşına kadar yapılan kontrollerinde komplikasyon saptanmadı.
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