The effects of iloprost on ischemia-reperfusion injury have been studied on the skeletal, muscle, liver, myocardium, kidney, and spinal cord. However, no sufficient data exist about effects of levosimendan on renal ischemia-reperfusion injury. The purpose of this experimental study was to investigate and compare effectiveness of levosimendan and iloprost on renal injury induced by ischemia and reperfusion. Fifty rabbits were divided into five groups. Levosimendan was continuously infused starting half an hour before the cross-clamp. Cross-clamp time was one hour. After one hour ischemia, levosimendan was continued for 4 h in Group A whereas Group B took iloprost in the same protocol. Group C was the control group which did not receive any medication. Group D was sham group and Group E was medicated both iloprost and levosimendan. Renal tissues were histologically and biochemically evaluated. The histological scores were obtained according to presence of tubular necrosis and atrophy, regenerative atypia, hydropic degeneration (Group A vs. Group C<0.001, Group B vs. Group C<0.001, Group D vs. Group C<0.01, Group E vs. Group C<0.001). Mean malondialdehyde levels were 114+/-12 nmol/g tissue; in Group A 121+/-13 nmol/g tissue, in Group B 134+/-13 nmol/g tissue, in Group E 130+/-11 nmol/g tissue, in Group D 134+/-11 nmol/g tissue (Group A vs. Group B; P=0.003, Group B vs. Group D; P=0.132, Group A vs. Group E; P=0.132). Malondialdehyde levels and histologic scores of all of the groups were significantly different from the control group. Iloprost and pentoxyfillin reduced renal ischemia-reperfusion injury in rabbit model. There was no significant difference between these two medications.
A case is reported of an inferior mesenteric artery aneurysm that starts approximately 1 cm from its origin and ends at the proximal portion of the bifurcation of the sigmoidal and left colic arteries accompanied with complete absence of the celiac axis and superior mesenteric arteries. Additionally, left renal artery stenosis existed. The diagnosis was made by digital subtraction arteriography and confirmed by magnetic resonance arteriogram. Disease involving the inferior mesenteric artery is extremely uncommon. This may be the first reported case of neurofibromatosis in combination with renal artery stenosis and inferior mesenteric artery aneurysm associated with celiac and superior mesenteric artery occlusion and treated surgically.
A AB BS ST TR RA AC CT T O Ob bj je ec ct ti iv ve e: : Angioneogenesis, which plays significant roles in a variety of physiological processes such as embryonic growth and wound healing, is strictly delimited and is finely tuned by a balance of proangiogenic and antiangiogenic factors. This study was conducted to investigate the angioneogenic effect of interleukin-8 (IL-8) administered intramuscularly. M Ma at te er ri ia al l a an nd d M Me et th ho od ds s: : Twelve New Zealand white rabbits were included in this study. A total daily dose of 4 micrograms (1 mcg/kg) of IL-8 was administered into the left (Group A) and saline solution into the right (Group B) gluteus maximus muscles of 6 rabbits for 6 days. The remaining 6 rabbits constituted the sham group (Group C). Gluteal muscle samples were obtained from injection sites in all groups and were stained with hematoxylin-eosin and immunohistochemically by using streptavidin biotin method with CD31 antibody. Avidin-biotin peroxidase method (LSAB) was used as secondary and binding antibody. Diaminobenzidine tetrahydrochloride (DAB) was used as chromogenic substance. In immunohistochemical staining with CD31, vascular channels covered with brown stained cells or cell clusters were considered and were counted as vascular network. R Re es su ul lt ts s: : Three subjects in Group A and one subject in Group B displayed findings of large muscle necrosis and regeneration. Vascular channel score was significantly higher in Group A (p=0.032) (Group A; median=12.5, min=6, max=16. Group B; median=5, min=4, max=13. Group C; median=4.5, min=4, max=13.) than in the other groups. C Co on nc cl lu us si io on n: : IL-8 chemokine family seems to stimulate angioneogenesis in rabbit skeletal muscles. This result is promising in terms of the possible therapeutic potential of IL-8. Daily administration at a dose of around 1 mcg/kg caused local tissue necrosis, hence use of alternative routes such as intraarterial administration must be investigated to avoid such complications. K Ke ey y W Wo or rd ds s: : Angioneogenesis; interleukin-8; skeletal muscle Ö ÖZ ZE ET T A Am ma aç ç: : Embriyonik büyüme ve yara iyileşmesi gibi bir dizi fizyolojik süreçte önemli rol oynayan anjiyogenez, proanjiyojenik ve antianjiyojenik faktörlerin birbirlerinden kesin çizgiler ile dengelenmesiyle ayrılmış ve hassas şekilde düzenlenmiştir. Bu çalışma, intramusküler olarak uygulanan interlökin-8 (IL-8)'in anjiyoneogenetik etkisini araştırmak için yapılmıştır. G Ge er re eç ç v ve e Y Yö ön nt te em ml le er r: : Çalışmada on iki adet Yeni Zelanda cinsi beyaz tavşan kullanıldı. Altı gün boyunca altı tavşanın sol gluteusuna 1 mcg/kg'dan toplam 4 mikrogram IL-8 (Grup A), sağ gluteusuna serum fizyolojik (Grup B) enjeksiyonu uygulandı. Diğer altı tavşan kontrol grubu idi (Grup C). Enjeksiyon uygulanan bölgelerden alınan gluteal kas örnekleri, Hemotoksilen-Eozin ve Streptavidin-biotin yöntemi kullanılarak CD31 antikoru ile immünohistokimyasal olarak boyandı. Avidin-biotin peroksidaz metodu (LSAB) ikincil ve bağla...
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