Artemisia cina is a medicinal plant species which produces bioactive compound potential to antitumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L -1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L -1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L -1 of 2,4-D combined with 1 of BA mg L -1 . The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.

<p>Viola cornuta L. is an ornamental plant that is primarily propagated by high-quality seeds that have high demand in subtropical countries. The use of fertilizers according to recommendations results in the growth of parent plants below the standard. Furthermore, seed production is generated below 2 g per plant and the seed germination capacity is below 80%. In addition, phosphorus (P) and potassium (K) nutrients can increase the growth and seed production of the plants, including ornamental plants. The study aimed to determine the influence of P and K nutrients on growth and seed production of V. cornuta L. Research was carried out in November 2018 - January 2019 at PT. Selektani Horticulture, Ngablak, Magelang. There were seven treatments with four replications. Experiments were arranged according to the Randomized Complete Block Design (RCBD). Plants were grown in 5l volume-polybags. Fertilization was done by watering the planting media. Analysis of variance was performed at 95% accuracy. The study shows that P and K nutrients have a highly significant effect on height, number of flowers, number of seed per capsule, seed production and germination energy and significantly affected the number of leaves. The addition of both P and K fertilizers in the vegetative and P in the generative stage (P6) can result in the productivity of 3.28 g plant-1 and produce germination energy ≥90%. It is recommended for increasing growth and seed production.</p>

Artemisinin is a secondary metabolite contained in the potential genus Artemisia as an antimalarial. Artemisia cina Berg ex Poljakov is a species from Artemisia that grows as a weed in Indonesia and contains artemisinin. The problem is that the artemisinin content in A. cina is minimal. The efforts must be made to increase the content. Clone improvement through breeding through genetic manipulation and enhancement of technical culture are the strategies that can use to improve the artemisinin content of A. cina. The purpose of this study was to determine the agronomic performance of nine A. cina polyploid genotypes (genotypes A, B, C, D, E, F, G, H, I) obtained from polyploidy induction using colchicines. The results of this study showed that leaf area and chlorophyll content were not significantly different among genotypes. In contrast, shoot dry weight, and plant height showed that I genotype was significantly higher than other genotypes. Glandular trichomes density of genotype was more elevated than different genotypes. Genotypes F, H, I had high artemisinin content compared to other genotypes. The genotype I showed higher artemisinin weight than other genotypes.

No abstract

No abstract