Aktivitas Enzim Kitinase pada Kacang Tanah yang Sehat dan yang Terinfeksi Sclerotium rolfsii

Pudjihartati, Endang; Siswanto,; Ilyas, Satriyas; Sudarsono, Sudarsono

doi:10.1016/s1978-3019(16)30385-0

Cited by 1 publication

(1 citation statement)

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“…Chitinase is a hydrolytic enzyme that can hydrolyze chitin in its β-1,4-glycosidic bonds by producing chitin derivatives such as chitin oligomers which have many benefits [5] Chitinase enzymes are currently widely used as biocontrol agents because they can degrade chitin into environmentally friendly products and can be used in the fields of health, food, industry and others Pratiwi et al (2015); [6] The decomposition process of lignocellulose compounds can involve microorganisms. According to Indrawati, 2005; Yosmar, Suharti, and Rasyid (2013), microorganisms involved in cellulose decomposition vary, including fungi, bacteria, and actinomycetes.Similar to [8] common chitinase is produced by bacterial cells, fungi, animals, and plants.…”

Section: Introductionmentioning

confidence: 99%

Screening Endophytic Fungi from Local Rice for Lignocellulolytic Enzyme Production

Syamsia¹,

Idhan²,

Hakim³

et al. 2019

Proceedings of the 1st International Conference on Science and Technology, ICOST 2019, 2-3 May, Makassar, Indonesia

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Lignocellulose is a complex molecule consisting of lignin, cellulose, and hemicellulose was difficult to degrade. Microorganisms such as fungi and bacteria have the ability to produce lignocellulolytic enzymes that can degrade lignocellulose. This study aimed to isolate and screen endophytic fungi isolates from local rice which have the ability to produce lignocellulolytic enzymes. Entophytic fungi were isolated from roots, stems, and leaves of local rice. Fungi was isolated on Potato dextrose Agar (PDA) media. Screening of endophytic fungi for lignocellulolytic enzyme production by Czapek Dox Agar (CDA) media which have been added with reamazol brilliant blue (RBB), chitin, carboxymotin cellulose, pectin and, lignin as much as 0.1% respectively. The presence of clear zones around endophytic fungi isolates is an indicator of the ability to produce chitin, cellulose, pectin, and lignin. Successfully isolated 16 endophytic fungi isolates from Pulu Mandoti Local rice. The 16 endophytic fungus isolates have the different ability in producing lignocellulosic enzymes (cellulose, chitin, lignin and pectin). There were 3 isolates that have the highest ability in producing cellulose, chitin, lignin and pectin enzymes. These results relevant to explore endophytic fungi and has potential in degrading agricultural waste and controlling pests and diseases on plants.

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Section: Introductionmentioning

confidence: 99%