Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.

No abstract

Artemisia cina is a medicinal plant species which produces bioactive compound potential to antitumor, antifungal and antibacterial medicines. This study was aimed to obtain A. cina polyploid plants through a treatment of growth regulators in shoot culture. The shoot were treated in 1; 15; 2; and 3 mg L -1 of 2,4-dichloro phenoxy acetic acid (2,4-D) combined with 1; 1.5; 2; 3 mg L -1 of Benzyladenine (BA) for 28 days. Chromosome analysis showed that the highest polyploidy percentage, 23%, was reached in the treatment of 2 mg L -1 of 2,4-D combined with 1 of BA mg L -1 . The polyploidy level varied, comprising 2n=3x, 2n=4x, 2n=5x, 2n=6x, with the highest polyploidy level percentage, 28.57%, and it was attained in the tetraploid (2n=4x). Polyploid plants had larger leaves area, larger stomatal size, and higher chlorophyll content than diploid plants. However stomatal density of polyploidy plants was lower than that of in diploid plants.

Penggunaan koagulan lateks yang tepat mampu menghasilkan karet bermutu baik. Namun, jaminan ketersediaan dan harga koagulan lateks anjuran menyebabkan petani karet beralih menggunakan koagulan non-anjuran. Penelitian ini mempelajari tentang mutu karet yang digumpalkan dengan koaglan anjuran asam format dan koagulan non-anjuran asam sulfat. Hasil penelitian menunjukkan bahwa bobot basah koagulum asam format lebih berat dibandingkan bobot basah koagulum asam sulfat, meskipun waktu koagulasi dengan menggunakan asam sulfat lebih cepat dibandingkan waktu koagulasi dengan bahan penggumpal asam format. Mutu karet yang dihasilkan oleh penggumpalan asam format berdasarkan parameter kadar karet kering, kadar abu dan kadar zat menguapnya lebih baik dibandingkan dengan mutu karet yang digumpalkan menggunakan asam sulfat.