IntroductionTumor detection and visualization plays a key role in the clinical workflow of a patient with suspected cancer, both in the diagnosis and treatment. Several optical imaging techniques have been evaluated for guidance during oncological interventions. Optical coherence tomography (OCT) is a technique which has been widely evaluated during the past decades. This review aims to determine the clinical usefulness of OCT during cancer interventions focussing on qualitative features, quantitative features and the diagnostic value of OCT.MethodsA systematic literature search was performed for articles published before May 2018 using OCT in the field of surgical oncology. Based on these articles, an overview of the clinical usefulness of OCT was provided per tumor type.ResultsA total of 785 articles were revealed by our search, of which a total of 136 original articles were available for analysis, which formed the basis of this review. OCT is currently utilised for both preoperative diagnosis and intraoperative detection of skin, oral, lung, breast, hepatobiliary, gastrointestinal, urological, and gynaecological malignancies. It showed promising results in tumor detection on a microscopic level, especially using higher resolution imaging techniques, such as high-definition OCT and full-field OCT.ConclusionIn the near future, OCT could be used as an additional tool during bronchoscopic or endoscopic interventions and could also be implemented in margin assessment during (laparoscopic) cancer surgery if a laparoscopic or handheld OCT device will be further developed to make routine clinical use possible.
Acousto-optic imaging is based on light interaction with focused ultrasound in a scattering medium. Thanks to photorefractive holography combined with pulsed ultrasound, we perform a time-resolved detection of ultrasound-modulated photons in the therapeutic window (780 nm). A high-gain SPS:Te crystal is used for this purpose and enables us to image through large optical thickness (500 mean free paths). We are able to generate three-dimensional (3D) acousto-optic images by translating a multielement ultrasound probe in only one direction. A 3D absorbing object is imaged through a 3 cm thick phantom.
Histopathological examination of temporal artery biopsy (TAB) remains the gold standard for the diagnosis of giant cell arteritis (GCA) but is associated with essential limitations that emphasize the need for an upgraded pathological process. This study pioneered the use of full-field optical coherence tomography (FF-OCT) for rapid and automated on-site pathological diagnosis of GCA. Sixteen TABs (12 negative and 4 positive for GCA) were selected according to major histopathological criteria of GCA following hematoxylin-eosin-saffronstaining for subsequent acquisition with FF-OCT to compare structural modifications of the artery cell wall and thickness of each tunica. Gabor filtering of FF-OCT images was then used to compute TAB orientation maps and validate a potential automated analysis of TAB sections. FF-OCT allowed both qualitative and quantitative visualization of the main structures of the temporal artery wall, from the internal elastic lamina to the vasa vasorum and red blood cells, unveiling a significant correlation with conventional histology. FF-OCT imaging of GCA TABs revealed destruction of the media with distinct remodeling of the whole arterial wall into a denser reticular fibrous neo-intima, which is distinctive of GCA pathogenesis and accessible through automated Gabor filtering. Rapid on-site FF-OCT TAB acquisition makes it possible to identify some characteristic pathological lesions of GCA within a few minutes, paving the way for potential machine intelligence-based or even non-invasive diagnosis of GCA.
Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.
Collagen and its derivative proteins have been widely used as a major component for cosmetic formulations as a natural ingredient and moisturizer. Most commercially available collagens are animal-derived collagen type I and other forms of collagen, such as type III collagen, are far less prevalent in animals, making extraction and purification extremely difficult and expensive. Here, we report the production of a 50 kDa protein produced in yeast that is 100% identical to the N-terminus of the human type III collagen. This recombinant protein has a larger molecular weight than most incumbent recombinant collagen proteins available for personal care applications. We report the industrialization of both the fermentation and purification processes to produce a final recombinant protein product. This final protein product was shown to be safe for general applications to human skin and compatible with common formulation protocols, including ethanol-based formulations. This recombinant collagen type III protein was also shown to uniquely stimulate both collagen type I and type III production and secretion by primary human dermal fibroblasts. The unique combination of biostimulation, compatibility with beauty product formulations and demonstrated commercial production, make this novel recombinant type III collagen a good candidate for broad application in the cosmetics industry.
DFFOCT allows for the visualization and characterization of cellular dynamics, providing future direction for cell-targeted therapeutics. In this work, we analyze the potential of DFFOCT to increase understanding of dynamic cellular processes under varying conditions.
August Wilson's The Piano Lesson features a debate between an African American brother and sister over the ownership of a richly symbolic piano, a family heirloom that represents the Charles family's slave heritage and its endurance through Reconstruction. Ownership questions like the one presented in The Piano Lesson can usually be resolved in the courts, but Wilson's play suggests that the law might be unable to resolve property disputes so problematically entangled with the legacy of slavery. Wilson offers, instead, a non-legal resolution to the piano debate presented in his play. August Wilson's The Piano Lesson and the Limits of Law I. Introduction At the heart of The Piano Lesson, August Wilson's 1990 Pulitzer Prize-winning play, is a debate between an African-American brother and sister over the rightful ownership of a piece of property. The piano sitting in Berniece's home in Pittsburgh in 1936 has been held by the Charles family, or by the white Sutter family, who once owned the Charleses, since 1856, when Robert Sutter, a Mississippi plantation owner, traded two Charles family slaves, Mama Berniece and her nine-year-old son Walter, for the piano as an anniversary present for his wife Ophelia. Soon, however, Ophelia missed the two departed slaves and felt guilty about their sale, perhaps because the transaction broke up a functioning slave family. To assuage Ophelia's guilt, a remaining Sutter slave, Papa Boy Willie, Mama Berniece's husband and a skilled wood sculptor, carved the images of his wife and his son into the frame of the piano. Papa Boy Willie continued to carve into the piano important events from his family's history-his marriage, his mother's funeral, the birth of his son, the sale of his wife and son. The piano stayed in possession of the Sutter family after emancipation; but on July 4, 1911, Boy Charles, the grandson of the wood carver and Berniece's father, stole the piano from the Sutters. When Robert Sutter's son discovered the theft, he killed Boy Charles by setting fire to a yellow train boxcar in which Boy Charles was hiding. Berniece took the piano with her when she migrated north from Mississippi in 1933. A widow, Berniece lives in Pittsburgh with her daughter Maretha and her Uncle Doaker. The debate between Berniece and her older brother Boy Willie is over the sale of the piano. Boy Willie and his friend Lymon have traveled to Pittsburgh from Mississippi with a
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