DFFOCT As a Tool to Detect Cellular Activity

Abstract

DFFOCT allows for the visualization and characterization of cellular dynamics, providing future direction for cell-targeted therapeutics. In this work, we analyze the potential of DFFOCT to increase understanding of dynamic cellular processes under varying conditions.

Methods and applications of full-filed optical coherence tomography: a review

“…The ability of FF-OCT to track the growth and development of cells in vitro can monitor the cell viability change in disease modeling or artificial tissue transplantation as well as reveal the mechanism of structural and functional reconstruction of 3D cultured tissues such as organoids and tumor microspheres, thereby facilitating research on the mechanism of human development and disease. 15 , 26 , 27 , 32 , 34 , 114 The functional imaging extension of FF-OCT is also applied in spectroscopic FF-OCT, with different pseudocolors showing melanocytes in ex vivo Xenopus laevis tadpole embryos at high resolution. 115 , 116 In addition, it also includes superimposing the fluorescence microscopic image of the mouse colon and the FF-OCT image, so more information can be displayed in one image; 117 the images obtained at the same time at the center wavelength of 800 and 1200 nm are given different colors, respectively.…”

“…As a type of optical microscopy, FF-OCT is advantageous in terms of resolution, penetration depth, imaging speed, noninvasiveness, and cost, but its application depends on the performance optimization of this technology, such as enhancing image reconstruction efficiency, optimizing image quality, extending imaging depth and field, and mining image information. In recent years, with the advancement of multimodal FF-OCT, particularly dynamic full-field optical coherence tomography (D-FFOCT), researchers can obtain the dynamic information of biological tissues and cells nondestructively, which further expands the application range of FF-OCT. 23 , 26 – 32 It is of significance to review the research progress and application of FF-OCT to further promote the development of 3D high-resolution imaging technology and solve the problem of nondestructive detection in microscopic scale.…”