Methods and applications of full-filed optical coherence tomography: a review

Fu, Rongzhen; Xu, Chen; Xu, Mingzhen

doi:10.1117/1.jbo.27.5.050901

Cited by 9 publications

(4 citation statements)

References 117 publications

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“…It also permits their respective dynamic contrast with an increased temporal resolution, thus opening access to subsequent acquisitions at different times. Such a report of in-depth D-FF-OCT-based monitoring of A. fumigatus dynamics is, to our knowledge, the first attempt to use low coherence interference microscopy for live-cell imaging in medical mycology ( Hickey and Read, 2009 ; Islam et al., 2017 ; Pfister et al., 2020 ; Wang et al., 2022 ).…”

Section: Discussionmentioning

confidence: 93%

“…D-FF-OCT relies on a specific kind of interference microscopy with a broadband light source. This provides both high-resolution and subcellular metabolic contrast imaging of biological tissues without the need for any tissue sample preparation or coloration step ( Apelian et al., 2016 ; Maldiney et al., 2020 ; Quénéhervé et al., 2021 ; Wang et al., 2022 ). When applied to the observation of different filamentous fungal species, D-FF-OCT makes it possible to identify multiple intracellular compartments with a definition approaching that of conventional microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in Aspergillus fumigatus

Maldiney,

Garcia-Hermoso,

Sitterlé

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionThe diagnosis of cutaneous manifestations of deep mycoses relies on both histopathological and direct examinations. Yet, the current diagnostic criteria cannot prevent missed cases, including invasive aspergillosis, which requires the development of a novel diagnostic approach and imaging tools. We recently introduced the use of dynamic full-field optical coherence tomography (D-FF-OCT) in fungal diagnostics with a definition approaching that of conventional microscopy and the ability to return metabolic information regarding different fungal species. The present work focuses on subcellular dynamics and live-cell imaging of Aspergillus fumigatus with D-FF-OCT to follow the fungal growth stages.MethodsThe A. fumigatus ATCC 204305 quality-control strain was used for all imaging experiments, following incubation times varying between 24 and 72 h at 30°C in a humidified chamber on Sabouraud dextrose agar. Fungal growth was subsequently monitored with D-FF-OCT for up to 5 h at room temperature and following the pharmacological stress of either voriconazole, amphotericin B, or caspofungin gradient concentration.ResultsD-FF-OCT images allow not only the visualization of intracellular trafficking of vacuoles but also an evolving dynamic segmentation of conidiophores depending on the chronological development and aging of the hyphae or the effect of antifungal treatment. The same applies to conidial heads, with the most intense D-FF-OCT signal coming from vesicles, revealing a changing dynamic within a few hours only, as well as complete extinction following subsequent drying of the Sabouraud dextrose agar.DiscussionThese results provide additional data on the ability of D-FF-OCT to monitor some of the main life cycle processes, dynamics, and intracellular trafficking of vacuoles in A. fumigatus, with or without the effect of pharmacological stress. Such complementary metabolic information could help both clinicians and microbiologists in either mechanistic studies toward experimental mycology or the development of a potential D-FF-OCT-guided diagnosis of superficial fungal infections.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in Aspergillus fumigatus

Maldiney,

Garcia-Hermoso,

Sitterlé

et al. 2023

Front. Cell. Infect. Microbiol.

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“…It is worth stating that the equations in this section are mainly used by TD-FF-OCT in human in vivo eye imaging, and may differ in detail for other uses of imaging, and the imaging equations in other scenarios and more details can be found in the references. 44,47 TD-FF-OCT first prevents defocus light from reaching the detector, then detects the entire bright field, and then relies on the rear demodulation scheme to generate the optical part. Each bright-field image on the camera contains: (1) ballistic single-scattered light from the sample optical section and reflected light from the reference mirror that interferes ('coherent' light) and (2) light that does not participate in interference ('incoherent' light) from the other layers of the sample, from the reference mirror, stray light reflected from optical components, as well as the multiply-scattered light from the sample optical section.…”

Section: Image Reconstructionmentioning

confidence: 99%

Time-domain full-field optical coherence tomography (TD-FF-OCT) in ophthalmic imaging

Zhang

Mazlin

Fei

et al. 2023

Therapeutic Advances in Chronic Disease

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Ocular imaging plays an irreplaceable role in the evaluation of eye diseases. Developing cellular-resolution ophthalmic imaging technique for more accurate and effective diagnosis and pathogenesis analysis of ocular diseases is a hot topic in the cross-cutting areas of ophthalmology and imaging. Currently, ocular imaging with traditional optical coherence tomography (OCT) is limited in lateral resolution and thus can hardly resolve cellular structures. Conventional OCT technology obtains ultra-high resolution at the expense of a certain imaging range and cannot achieve full field of view imaging. In the early years, Time-domain full-field OCT (TD-FF-OCT) has been mainly used for ex vivo ophthalmic tissue studies, limited by the low speed and low full-well capacity of existing two-dimensional (2D) cameras. The recent improvements in system design opened new imaging possibilities for in vivo applications thanks to its distinctive optical properties of TD-FF-OCT such as a spatial resolution almost insensitive to aberrations, and the possibility to control the curvature of the optical slice. This review also attempts to look at the future directions of TD-FF-OCT evolution, for example, the potential transfer of the functional-imaging dynamic TD-FF-OCT from the ex vivo into in vivo use and its expected benefit in basic and clinical ophthalmic research. Through non-invasive, wide-field, and cellular-resolution imaging, TD-FF-OCT has great potential to be the next-generation imaging modality to improve our understanding of human eye physiology and pathology.

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“…3 Although this measurement technique enables non-contact volumetric imaging, there are few applications in the technical field besides the use of TD-FF-OCT in ophthalmology 4 or in the biomedical field. 5 In this paper, we would like to introduce another potential application of TD-FF-OCT in technical fields, which focuses on the thickness measurement of foils. In this field, for example, Yao and Rolland 6 have investigated film thickness measurement by OCT, but with a scanning OCT system.…”

Section: Introductionmentioning

confidence: 99%

Construction of a time-domain full-field OCT for non-contact volumetric layer thickness measurement

Bulun

Lotz

Keksel

et al. 2023

Optical Measurement Systems for Industrial Inspection XIII

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In this paper the design of a Time-Domain Full-Field OCT (TD-FF-OCT) setup for non-contact volumetric layer thickness measurement is presented and quantified in terms of achievable accuracy and performance. The capabilities of the instrument regarding its measuring accuracy are verified using foil thickness standards of different strength. Afterwards, a technical application of measuring a thin and rough varnish coated PET foil (foil thickness t f oil ≈ 150 µm, rough varnish layer thickness t varnish ≈ 10 µm) is carried out. Since the device is designed to conduct areal measurements, the thickness can be accurately determined over several measurement points. The results are compared with results achieved by applying an alternative but destructive and more time-consuming measuring method (evaluation of microscopic images of respective foil slices produced by using a microtome). Finally, the achievements are summarized and identified optimization potential is highlighted.

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Methods and applications of full-filed optical coherence tomography: a review

Cited by 9 publications

References 117 publications

Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in Aspergillus fumigatus

Dynamic full-field optical coherence tomography for live-cell imaging and growth-phase monitoring in Aspergillus fumigatus

Time-domain full-field optical coherence tomography (TD-FF-OCT) in ophthalmic imaging

Construction of a time-domain full-field OCT for non-contact volumetric layer thickness measurement

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