Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy

Abstract

Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.

Dynamic contrast optical coherence tomography (DyC-OCT) for label-free live cell imaging

“…To create a colormap, the integrated intensity within different frequency bands is color-coded 25 – 27 ; for example, signals in the 0.8–4 Hz range are shown in green, while signals in the 4–20 Hz range are shown in red. Furthermore, PSD can highlight dominant frequencies using a continuous colormap 29 , 30 . These colormaps can be overlaid on structural OCT images to provide a co-registered display of tissue dynamics (Fig.…”

Methods and applications of full-filed optical coherence tomography: a review

“…The ability of FF-OCT to track the growth and development of cells in vitro can monitor the cell viability change in disease modeling or artificial tissue transplantation as well as reveal the mechanism of structural and functional reconstruction of 3D cultured tissues such as organoids and tumor microspheres, thereby facilitating research on the mechanism of human development and disease. 15 , 26 , 27 , 32 , 34 , 114 The functional imaging extension of FF-OCT is also applied in spectroscopic FF-OCT, with different pseudocolors showing melanocytes in ex vivo Xenopus laevis tadpole embryos at high resolution. 115 , 116 In addition, it also includes superimposing the fluorescence microscopic image of the mouse colon and the FF-OCT image, so more information can be displayed in one image; 117 the images obtained at the same time at the center wavelength of 800 and 1200 nm are given different colors, respectively.…”

“…As a type of optical microscopy, FF-OCT is advantageous in terms of resolution, penetration depth, imaging speed, noninvasiveness, and cost, but its application depends on the performance optimization of this technology, such as enhancing image reconstruction efficiency, optimizing image quality, extending imaging depth and field, and mining image information. In recent years, with the advancement of multimodal FF-OCT, particularly dynamic full-field optical coherence tomography (D-FFOCT), researchers can obtain the dynamic information of biological tissues and cells nondestructively, which further expands the application range of FF-OCT. 23 , 26 – 32 It is of significance to review the research progress and application of FF-OCT to further promote the development of 3D high-resolution imaging technology and solve the problem of nondestructive detection in microscopic scale.…”

Label-free intratissue activity imaging of alveolar organoids with dynamic optical coherence tomography

“…Several DOCT methods have been demonstrated to visualize the intratissue and intracellular activities through the fluctuation magnitude [ 28 – 30 ], time-frequency spectrum [ 28 , 31 – 34 ], and time-correlation property [ 30 , 35 , 36 ] of OCT signals. DOCT has been applied to a variety of samples including human esophageal and cervical biopsies [ 34 ], ex vivo mouse or rat organs [ 29 , 31 , 33 , 36 – 38 ], in vivo zebrafish [ 39 ], in vitro cell culture [ 40 ], spheroid [ 30 , 41 ], mammaria organoid [ 42 ], and retinal organoid [ 43 ]. Particularly for pulmonary tissues, Ling et al.…”