The aim of these investigations was to study vitamin E supplement effect in male albino rats after 30 days of repeated treatment. Four groups of six male rats were orally administered distilled water (control), 500, 1000 and 2000 mg/kg body weight vitamin E daily for 30 days. The impact of the treatment on percent body weight and mortality was determined and compared to the control group. Some hematological analysis, biochemical parameters and histological examination of different body organs were assessed. The rats treated with different doses of vitamin E supplement showed no deaths recorded in 30 days. The treatment with higher dose Vitamin E supplementation" caused significant alteration at the hematological, biochemical and histological level. Therefore, oral administration of vitamin E supplement in rats for 30 days was not safe for the liver and kidney and in the other hand, safe for the testes therefore that side effect on the liver and kidney should be considered when recommended vitamin E for therapeutic purpose. Care should be taken in taking high doses of vitamin E.
Vitamin E (α-tocopherol) is a potent antioxidant and possesses an important role in deactivating free radicals in the cells and preventing the hazardous effects of reactive oxygen species thus stopping the lipid peroxidation process. However, the overdosing of a vitamin is toxic and leads to many harmful effects. The current work aimed to assess the potential subchronic toxicity of vitamin E megadoses for 90 days of treatment and after 45 days of recovery. Forty-seven male albino rats weighting (120-140 g) were used in this study and were divided into seven groups each of seven animals. The normal control group, three groups treated with (500, 1000, and 2000 mg/kg) of vitamin E for successive 90 days, and the last three groups were treated with the same doses and left 45 days for recovery. The results showed that higher doses of vitamin E had significantly increased liver enzymes ALT and AST, MDA, and altered hematological parameters. The toxicity was proportional to the dose and recovery period showed maintained liver toxicity while kidney regains their normal function. The study concluded that although vitamin E reported being a potent antioxidant, it is very toxic unless it had used within the range of recommended doses and should be used under the prescription of the physicians
Blood samples were obtained from 28 mature healthy male Muscovy ducks for screening of a partial sequence of Growth hormone gene (GH gene) for single nucleotide polymorphisms using PCR amplification of the extracted DNA. The results of our research revealed one locus of exon 2 of growth hormone gene and its partial flanking intron. Polymorphisms in this locus were investigated by using direct sequencing and single strand conformational polymorphism (SSCP) technique. One unique pattern of SSCP was detected. Nucleotide sequencing was performed to endorse the current result which proved the absence of single nucleotides' polymorphisms (SNPs) or any other type of polymorphisms in the studied locus. The DNA sequencing and PCR-SSCP patterns confirmed that there was no polymorphism in all studied birds. In turn, it is spelled that PCR-SSCP failed to produce electrophoresis patterns capable of discriminating between the GH gene loci in Muscovy duck breed revealing no polymorphism in all studied individuals. Also, we compared our sequences with the published sequence on the gene bank of GH gene in Mallard ducks under the following accession number AB158762.1. Three SNPs were detected between Muscovy and Mallard breeds which are (94 A-G, 271 C-T and 298 G-A) these SNPs led to change of two amino acids proline and valine amino acids in GH protein in mallard ducks into serine and isoleucine in Muscovy ducks. In conclusion, these changes in the nucleotide sequence, amino acid and protein chain may be the main cause of differences of the phenotypic features, productive and reproductive traits between Muscovy and mallard duck breeds.
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