Study of the toxic effect and safety of vitamin E supplement in male albino rats after 30 days of repeated treatment

El-Hak, Heba Nageh Gad; ELaraby, Eman E.; Hassan, Ahmed K.; Abbas, Osama A.

doi:10.1016/j.heliyon.2019.e02645

Cited by 19 publications

(12 citation statements)

References 55 publications

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“…This study is in disagreement with the work of Akinloye et al [13] which reported increased white cell count. The lack of discrepancy in the white cell count and differentials (neutrophils and lymphocyte) is an evidence of null alteration of the immune system with no inflammatory trigger to bring about a visible reaction as noted in other studies [14][15][16].…”

Section: Discussionsupporting

confidence: 56%

Synergistic Effect of Vitamin E and C Treatment on Paraquat Induced Haemotoxicity in Rattus norvegicus

Okolonkwo

Nyebuchi

Adjekuko

et al. 2022

AIR

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Paraquat is a herbicide, commonly used in the agricultural practices to prevent weed infestation. The Environmental Protection Agency in some countries has placed a restriction on paraquat and there is no identified record of chelating agent or antidote for paraquat. Oxidation of paraquat results in the of superoxides formation. Superoxides generation causes cellular damage. Vitamin C (ascorbic acid) is a water soluble vitamin, with antioxidant potential. It is efficient in clearing free radicals, including hydroxyl radicals, peroxyl radicals and superoxide anion. E is a fat soluble vitamin but considered to be relatively safe when compared to other fat-soluble vitamins Empirical evidence has shown therapeutic efficacy of vitamin E hence its common use in therapeutics. Our goal is to determine the potency of combined effect of vit. E + C on paraquat induced toxicity in male albino rats. As much as 200 rats were obtained and parted into four main groups of 50 rats each. The groups were A, B C and D. The “A” group was not induced with paraquat; groups “B”, “C”, and “D” was induced every two weeks with 0.02g, 0.04g, and 0.06g of paraquat per kg of rat respectively for three months. Each of the main groups had subgroups. “A” group had “A0” and “AVEC” subgroups; “B” group had “B0” and “BVEC” subgroups; “C” group had “C0” and “CVEC” subgroups; “D” group had “D0” and “DVEC” subgroups. “A0”, “B0”, “C0” and “D0” subgroups were treated with paraquat only, while “AVEC”, “BVEC”, “CVEC” and “DVEC” were treated orally with 500mg of vitamin E and 2000mg/l of vitamin C medicated water every week. Comparison of intergroups A0, B0, C0 and D0 was statistically significant, p-value<0.05 in Hb and PCV. There was no statistical difference with respect to T-WBC, neutrophil, and lymphocytes across the groups. AVEC, BVEC, CVEC and DVEC intergroup comparison was statistically significant, p-value<0.05 in Hb and PCV, whereas in comparison with T-WBC, neutrophil, and lymphocytes were not statistically significant among the groups. The finding from this study has shown that a combination of vitamin E and C therapy is potent against paraquat toxicity in male albino rats on one month of weekly treatment and can be used to treat the condition on a weekly basis.

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Section: Discussionsupporting

confidence: 56%

Synergistic Effect of Vitamin E and C Treatment on Paraquat Induced Haemotoxicity in Rattus norvegicus

Okolonkwo

Nyebuchi

Adjekuko

et al. 2022

AIR

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“…The inhibition of the protective activity of GST-π could be the cause of tumour-promoting effect of vitamin E in mice ( Mitchel and McCann, 1993 ). El-Hak et al (2019) studied the effects of vitamin E supplements (daily 500, 1,000, and 2,000 mg vitamin E/kg body weight) in male albino rats. Treatment with higher doses of vitamin E caused significant changes in haematology, biochemistry and histological damage, including the kidneys.…”

Section: Adverse Effects Of Vitamin E On the Kidneys In Rodent Studiesmentioning

confidence: 99%

Beneficial and adverse effects of vitamin E on the kidney

Baltušnikienė

Stanevičienė²,

Jansen³

2023

Front. Physiol.

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This article reviews the beneficial and adverse effects of high-dose vitamin E supplementation on the vitamin E status and renal function in human and rodent studies. The high doses of vitamin E, which can cause renal effects, were compared to upper limits of toxicity (UL) as established by various authorities worldwide. In recent mice studies with higher doses of vitamin E, several biomarkers of tissue toxicity and inflammation were found to be significantly elevated. In these biomarker studies, the severity of inflammation and the increased levels of the biomarkers are discussed together with the need to re-evaluate ULs, given the toxic effects of vitamin E on the kidney and emphasizing oxidative stress and inflammation. The controversy in the literature about vitamin E effects on the kidney is mainly caused by the dose-effects relations that do not give a clear view, neither in human nor animals studies. In addition, more recent studies on rodents with new biomarkers of oxidative stress and inflammation give new insights into possible mechanisms. In this review, the controversy is shown and an advice given on the vitamin E supplementation for renal health.

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“…When the rats were treated with different doses of vitamin E over a period of 30 days, their kidneys and livers were affected, but proved it has no adverse effect on the testes. As such, El-Hak et al ( 36 ) recommended exercising caution when taking high doses of vitamin E. Biological and histological modifications that appear due to exposure of mice to CPF (chlorpyrifos) during gestation and lactation were reversed by an oral supplementation of vitamin E, either to the dams or to their pups ( 37 ).…”

Section: Fat-soluble Vitamins E and Kmentioning

confidence: 99%

E, K, B5, B6, and B9 vitamins and their specific immunological effects evaluated by flow cytometry

et al. 2023

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It has been proven that vitamins play an essential role in preventing certain diseases since ancient times. It is thus fruitless to approach the roles of vitamins without making reference to the techniques used in evaluating the effects of these micronutrients. Therefore, the aim of this paper was to summarize the immunological effects of E, K, B5, B6, and B9 vitamins evaluated by flow cytometry. Some of these significant effects were presented and discussed: (a) The role of vitamins E in the prevention and treatment of different types of cancer. (b) The properties of K vitamins in the development and maintenance of pheochromocytoma Cell Line 12 (PC12) cells in Parkinson’s disease; (c) The improvement effect of vitamin B5 on the loss of bone mass in low estrogen conditions; (d) The anticancer role of vitamins B6. (e) The role of Vitamin B9 in the regulation of Treg cells. As such, the flow cytometry technique used to assess these properties is essential to evaluate the immunomodulatory effects of certain vitamins. The technique undergoes constant improvement which makes it possible to determine several parameters with a role in the modulation of the immune function and at the same time increase the accuracy of the methods that highlight them.

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Study of the toxic effect and safety of vitamin E supplement in male albino rats after 30 days of repeated treatment

Cited by 19 publications

References 55 publications

Synergistic Effect of Vitamin E and C Treatment on Paraquat Induced Haemotoxicity in Rattus norvegicus

Synergistic Effect of Vitamin E and C Treatment on Paraquat Induced Haemotoxicity in Rattus norvegicus

Beneficial and adverse effects of vitamin E on the kidney

E, K, B5, B6, and B9 vitamins and their specific immunological effects evaluated by flow cytometry

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