Evaluate Subchronic Toxic Effects of Repeated Exposure of Megadoses of Vitamin E in Male Albino Rats

Abstract

Vitamin E (α-tocopherol) is a potent antioxidant and possesses an important role in deactivating free radicals in the cells and preventing the hazardous effects of reactive oxygen species thus stopping the lipid peroxidation process. However, the overdosing of a vitamin is toxic and leads to many harmful effects. The current work aimed to assess the potential subchronic toxicity of vitamin E megadoses for 90 days of treatment and after 45 days of recovery. Forty-seven male albino rats weighting (120-140 g) were used in this study and were divided into seven groups each of seven animals. The normal control group, three groups treated with (500, 1000, and 2000 mg/kg) of vitamin E for successive 90 days, and the last three groups were treated with the same doses and left 45 days for recovery. The results showed that higher doses of vitamin E had significantly increased liver enzymes ALT and AST, MDA, and altered hematological parameters. The toxicity was proportional to the dose and recovery period showed maintained liver toxicity while kidney regains their normal function. The study concluded that although vitamin E reported being a potent antioxidant, it is very toxic unless it had used within the range of recommended doses and should be used under the prescription of the physicians