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Contribution/ OriginalityThis study is emphasizing the adverse effects of the synthetic food colorants on human health as previously reported in the literature.

Hairy root culture is a promising alternative method for the production of secondary metabolites. In this study, transformed root of Linum usitatissimum was established using Agrobacterium rhizogenes A4 strain from root cultures for lignans, phenolic acids and antioxidant capacity determination. Total lignin content (secoisolariciresinol diglucoside, secoisolariciresinol and matairesinol) was 55.5% higher in transformed root cultures than in the non-transformed root culture. Secoisolariciresinol was detected in higher concentration (2.107 μmol/g DM) in the transformed root culture than non-transformed culture (1.099 μmol/g DM). Secoisolariciresinol diglucoside and matairesinol were exclusively detected in the transformed root culture, but were not found in the non-transformed root culture. The overall production of phenolic acids in transformed roots was approximately 3.5 times higher than that of the corresponding non-transformed culture. Free radical scavenging DPPH˙ and ABTS˙ assays showed 2.9-fold and 1.76-fold higher anti-oxidant activity in transformed root culture as compared to non-transformed.

RESUMEN Semillas de alubias blancas y piel y semillas de granadas como agentes hipercolesterolémicos e hipolipidémicos en ratas albinasSe ha llevado a cabo un estudio para evaluar los efectos de las semillas de alubias blancas y de las de granadas así como de sus cáscaras secas y sus mezclas sobre los perfiles de lípidos de ratas que sufren de hiperlipidemia e hipercolesterolemia. La composición química de las alubias blancas y de las granadas (semillas y cáscaras) fue determinada en base a materia seca alcanzando una buena relación de proteí-nas, lípidos, fibra cruda y fenoles. Además, el contenido de fenoles de las semillas y las cáscaras de granada mostraron 16 compuestos que varían en cantidad entre ellos. Se observó que la catequina y los fenoles son los compuestos predominantes. Los resultados obtenidos mostraron un buen poder hipolipidémico de las semillas de alubias blancas y de granadas (semillas y las cáscaras), así como sus mezcla. Las semillas de alubias blancas en la dieta produjo una mejora general de la situación clínica, del perfil de lípidos en sangre (lípidos totales, triglicéridos, colesterol, HDL-C, LDL-C y VLDL-c), de la función hepática (ALT, AST y bilirrubina), de la función de los riñones (ácido úrico, urea y creatinina), de la peroxidación de lípidos en sangre y de las enzimas antioxidantes (SOD y CAT) por lo que se reduce la hiperlipidemia e hipercolesterolemia. La dieta mezcla presentó la mayor influencia sobre los estudios biológicos en relación con los otros tratamientos que utilizan solamente semillas de alubias blancas o granadas (semillas y cáscaras). PALABRAS CLAVE: Alubia blanca (Phaseolus vulga ris L.) -Granada (Punica granatum) -Hipocolesterolemia -Hipolipidemia. ABSTRACT White Bean seeds and Pomegranate peel and fruit seeds as hypercholesterolemic and hypolipidemic agents in albino ratsThis study was carried out to evaluate the effects of soaked white bean seeds, pomegranate seeds and dried peels and their mixtures on the lipid profiles of rats suffering from hyperlipidemia and hypercholesterolemia. The chemical compositions of dried soaked beans and pomegranate (seeds and peels) were determined on dry matter which amounted to good values for proteins, lipids, crude fiber and phenols. Also, the phenol contents of pomegranate seeds and peels showed 16 compounds varying in amount between them. It was noticed that catechin and phenol are the dominating compounds.The obtained results showed a good hypolipidemic ability for soaked bean seeds and pomegranate (seeds, peels and their mixtures) as well as their mixtures. A bean seeds diet produced a general improvement in the clinical status of blood lipid profile (total lipids, triglycerides, cholesterol, HDL-c, LDL-c and VLDL-c), liver function (ALT, AST and bilirubin), kidneys function (uric acid, urea and creatinine), blood lipid peroxidation and antioxidant enzymes (SOD and CAT) by which hyperlipidemia and hypercholesterolemia were reduced. The mixed diet had the best influence concerning biological studies than the other treatme...