No abstract

Serum folate and MTHFR C677T and MTR A2576G gene polymorphisms were the determinants for tHcy levels. The interaction between low levels of serum Cbl and MTHFR (C677T or A1298C) or MTRR A66G gene polymorphisms was associated with increased tHcy.

A avaliação do estado nutricional de ferro em indivíduos e populações tem sido alvo de muitas pesquisas científicas, uma vez que existem algumas questões ainda indefinidas. Através de um levantamento bibliográfico, mediante consulta às bases de dados Medline, Lilacs e Dedalus, foram selecionadas publicações científicas em português e inglês, no período de 1972 a 1998, que se referiam aos parâmetros hematológicos e bioquímicos utilizados na avaliação do estado nutricional de ferro. Os parâmetros disponíveis refletem os três diferentes estágios da carência de ferro, a qual se manifesta de forma gradual e progressiva no organismo, até o desenvolvimento da anemia ferropriva. De forma geral, não possuem boa sensibilidade e especificidade quando considerados isoladamente, apresentando vantagens e limitações que devem ser examinadas no momento da escolha. No sentido de se aumentar a sensibilidade e a especificidade do diagnóstico do estado nutricional de ferro, têm-se utilizado combinações dos diferentes parâmetros disponíveis, considerando-se a contribuição de cada um, de acordo com as características dos indivíduos estudados, as facilidades metodológicas e o custo do processo.

No abstract

Lower maternal cobalamin concentrations are associated with higher tHcy and lower SAM:SAH in newborns. Because SAM:SAH is closely linked with the activity of numerous enzymatic methylation reactions, these results suggest that methylation could be impaired in cobalamin-deficient pregnant women and their newborns.

Background The effects of high dose folic acid (FA) supplementation in healthy individuals on blood folate concentrations and immune response are unknown. Objective The aim of the study was to evaluate the effects of daily consumption of a tablet containing 5 mg of FA on serum folate; number and cytotoxicity of natural killer (NK) cells; mRNA expression of dihydrofolate reductase (DHFR), methylenetetrahydrofolate reductase (MTHFR), interferon-γ (IFNG), tumor necrosis factor-α (TNFA) and interleukin-8 (IL8) genes; and concentrations of serum inflammatory markers. Methods This prospective clinical trial was conducted in 30 healthy Brazilian adults, (15 women), aged 27.7 y (95% CI: 26.4, 29.1 y), with a body mass index (in kg/m2) of 23.1 (95% CI: 22.0, 24.3). Blood was collected at baseline and after 45 and 90 d of intervention. Serum folate concentrations were measured by microbiologic assay and HPLC-tandem mass spectrometry [folate forms, including unmetabolized folic acid (UMFA)]. We used real-time polymerase chain reaction to assess mononuclear leukocyte mRNA expression and flow cytometry to measure the number and cytotoxicity of NK cells. Results Serum folate concentration increased by ~5-fold after the intervention (P < 0.001), and UMFA concentrations increased by 11.9- and 5.9-fold at 45 and 90 d, respectively, when compared with baseline (P < 0.001). UMFA concentrations increased (> 1.12 nmol/L) in 29 (96.6%) participants at day 45 and in 26 (86.7%) participants at day 90. We observed significant reduction in the number (P < 0.001) and cytotoxicity (P = 0.003) of NK cells after 45 and 90 d. Compared with baseline, DHFR mRNA expression was higher at 90 d (P = 0.006) and IL8 and TNFA mRNA expressions were higher at 45 and 90 d (P = 0.001 for both). Conclusion This noncontrolled intervention showed that healthy adults responded to a high dose FA supplement with increased UMFA concentrations, changes in cytokine mRNA expression, and reduced number and cytotoxicity of NK cells. This trial was registered at www.ensaiosclinicos.gov.br as RBR-2pr7zp.

Genetic variations in GPX1 and SELENOP genes are associated with different responses of molecular and biochemical biomarkers of Se status after Brazil nut supplementation in healthy Brazilians. The SU.BRA.NUT study was registred at www.clinicaltrials.gov as NCT 03111355.

No abstract