BackgroundBrazilian berry is a fruit popularly known as “Jaboticaba,” rich in bioactive compounds with antioxidant and anti‐inflammatory properties. Senescence and overweight are increasing worldwide and are considered risk factors to prostatic pathogenesis mainly due to oxidative and inflammatory processes induction. Thus, this study aimed to evaluate the effect of two increasing doses of the patented jaboticaba peel extract (PJE) on oxidative‐stress and inflammation in the prostate of aging or high‐fat‐fed aging mice.MethodsPJE and/or high‐fat diet (HFD) treatments started with 11‐month‐old mice and lasted 60 days. The levels or the immunoexpression of different inflammatory (nuclear factor κB [NFκB], CD3+, cyclooxygenase 2 [COX‐2], toll‐like receptor 4 [TLR4], phosphorylated signal transducers and activators of transcription 3 [pSTAT‐3], tumor necrosis factor α [TNF‐α], interleukin 6 [IL‐6], and IL‐1β) and oxidative‐stress (catalase, superoxide dismutase 2 [SOD2], glutathione reductase [GSR], reduced glutathione, and glutathione peroxidase 3 [GPx3]) related molecules were analyzed by western‐blotting, immunohistochemistry, and enzyme‐linked immunosorbent assays.ResultsBoth PJE doses reduced the levels of oxidative‐stress‐related molecules (GPx3, GSR, catalase), lipid peroxidation (4‐hydroxynonenal), inflammatory mediators (COX‐2, TNF‐α, and pSTAT‐3) and CD3+ T cells number, which were associated with the maintenance of the glandular morphological integrity in aging and HFD‐fed‐aging mice. Nevertheless, only the high PJE dose reduced the NFκB and TLR4 levels in aging mice; and SOD2, IL‐6, and IL‐1β levels in HFD‐aging mice. Aging itself promoted an oxidative inflammation in the prostate, interfering in the levels of the different oxidative‐stress, lipid peroxidation, and inflammatory mediators evaluated, in association with high incidence of prostate epithelial and stromal damages. The HFD intake intensified aging alterations, showing an unfavorable prostatic microenvironment prone to oxidative and inflammatory damages.ConclusionsPJE exerted a dose‐dependent effect controlling inflammation and oxidative‐stress in aging and HFD‐fed aging mice prostate. This fact contributed to prostate microenvironment balance recovery, preserving the tissue architecture of this gland. Thus, the PJE emerges as a potential therapy to prevent inflammation and oxidative stress in the prostate.
O objetivo do presente artigo foi identificar os principais fatores associados à fratura radicular vertical e, apresentar os principais métodos diagnósticos com ênfase na tomografia computadorizada, e comparar estudos quantitativos in vitro e in vivo, referente à sensibilidade, especificidade e precisão da técnica diagnóstica. Foi realizada uma busca por artigos publicados entre 2011-2021 nas bases de dados Lilacs e Pubmed. Dos 446 registros encontrados, 38 artigos foram eleitos para fins de revisão bibliográfica intergativa. Em relação aos sinais e sintomas, dor, fístula, abcesso ou inchaço periodontal, e radiolucências periapicais e halo em forma de J são achados comuns. A tomografia computadorizada de feixe cônico foi evidenciada como método não-invasivo mais adequado para a detecção da fratura radicular. A técnica se destaca pela produção de imagens 3D de alta resolução que permitem a visualização precisa de linhas de fraturas. Em dentes com preenchimento por materiais radiopacos, tais como guta-percha e pinos metálicos, é comum ocorrerem distorções com produção de artefatos, que diminuem significativamente o poder diagnóstico.
The Brazilian berry, also known as jaboticaba, has a great antioxidant and anti-inflammatory potential, besides demonstrating positive effects on hormonal regulation and weight loss. Nowadays, both aging and overweight are considered public health issues, promoting metabolic and hormonal changes that have a substantial role in prostate injury. We demonstrated herein that a low dose of jaboticaba peel extract (PJE) is enough to limit the onset of damages and hormone receptor alterations on the anterior prostate in the senile or high-fat diet (HFD) groups. The senile mice (11-months old) received the PJE and/or a HFD for 60 days. The anterior prostates were collected for histopathological, immunohistochemistry and westernblotting analysis. The PJE treatment reduced the epithelium atrophy and inflammatory infiltrate frequencies besides decreasing the androgen receptor (AR); estrogen receptor alpha (ERα); and insulin-like growth factor 1 receptor (IGFR-1) immunoexpression; in the anterior prostate of both senile and HFD-senile mice. However, low prostatic intraepithelial neoplasia (PIN) frequency, reduced immunoexpression of stromal AR and epithelium IGFR-1 were only observed in the anterior prostate of the PJE and HFD-treated groups. HFD intake intensified the aging-induced histopathological and hormonal alterations by further increasing the AR, ERα and IGFR-1 immunoexpression, as well as the PIN lesion incidence in the anterior prostate. HIGHLIGHTS• Brazilian berry mitigate the disorder onset in prostate of senile/high-fat mice.• Brazilian berry reduced AR/ERα/IGFR-1 labeling in prostate of senile/high-fat mice.• Brazilian berry preventive effect increases according to the prostatic damages rise.
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