The Brazilian berry, also known as jaboticaba, has a great antioxidant and anti-inflammatory potential, besides demonstrating positive effects on hormonal regulation and weight loss. Nowadays, both aging and overweight are considered public health issues, promoting metabolic and hormonal changes that have a substantial role in prostate injury. We demonstrated herein that a low dose of jaboticaba peel extract (PJE) is enough to limit the onset of damages and hormone receptor alterations on the anterior prostate in the senile or high-fat diet (HFD) groups. The senile mice (11-months old) received the PJE and/or a HFD for 60 days. The anterior prostates were collected for histopathological, immunohistochemistry and westernblotting analysis. The PJE treatment reduced the epithelium atrophy and inflammatory infiltrate frequencies besides decreasing the androgen receptor (AR); estrogen receptor alpha (ERα); and insulin-like growth factor 1 receptor (IGFR-1) immunoexpression; in the anterior prostate of both senile and HFD-senile mice. However, low prostatic intraepithelial neoplasia (PIN) frequency, reduced immunoexpression of stromal AR and epithelium IGFR-1 were only observed in the anterior prostate of the PJE and HFD-treated groups. HFD intake intensified the aging-induced histopathological and hormonal alterations by further increasing the AR, ERα and IGFR-1 immunoexpression, as well as the PIN lesion incidence in the anterior prostate. HIGHLIGHTS• Brazilian berry mitigate the disorder onset in prostate of senile/high-fat mice.• Brazilian berry reduced AR/ERα/IGFR-1 labeling in prostate of senile/high-fat mice.• Brazilian berry preventive effect increases according to the prostatic damages rise.
The yellow passion‐fruit (passiflora‐edulis) is rich in piceatannol (PIC), a polyphenolic and resveratrol analog known by its beneficial effects on health such as anti‐inflammatory anti‐cancer properties. The present study aimed to investigate the passion‐fruit extract (PFE) mechanistic of action in prostate cancer (PCa) cell lines with different genetic backgrounds and in the transgenic adenocarcinoma of the mouse prostate (TRAMP). For in vitro experiments, LNCaP (androgen‐responsive) and PC‐3 (androgen‐independent) cells were treated with different doses of PFE (125–500 μg/mL) and used for cell viability assay and western blotting analysis. For in vivo experiments, TRAMP mice were gavaged with 20mg/Kg of PIC contained in the PFE during 4 or 10 weeks of treatment. The ventral prostate was collected for light microscopy, immunohistochemistry and western blotting analysis. Because inflammation is intrinsically evolved with prostate alterations, like hormonal imbalance and cell survival, we focused on if PFE could affect these pathways. PFE treatment caused a significant reduction of cell viability in both cell lines tested, as well as decreased nuclear factor kappa B (NFκB) and toll‐like receptor 4 protein level. Interestingly, LNCaP cells were more sensitive to the PFE action in all experiments and we verified that the androgen receptor was markedly reduced by the treatment in these cells. PFE delayed CaP progression in the TRAMP model in both periods of treatment, significantly decreasing high‐grade prostatic intraepithelial neoplasia and well‐differentiated adenocarcinoma. These results were confirmed by the decrease of proliferating cell nuclear antigen immunostaining. Also, treatment with PFE reduced inflammation markers such as tumor necrosis factor‐alpha (TNF‐α), NFκB, and modulated negatively cell cycle regulatory protein levels and signal transducer and activator of transcription 3. Therefore, the results herewith showed that PFE was able to modulate disease progression through the inhibition of the inflammatory pathways, delay of the cell cycle, especially in the hormone‐dependent profile, demonstrating its chemopreventive and therapeutic potential in PCa. Support or Funding Information Sao Paulo Research Foundation ( Number: 2017/01573‐5)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.