The vitamin requirements of 63 strains and the amino acid requirements of 40 strains belonging to the genus Leuconostoc have been determined. The amino acid requirements separate the genus into dextran and non-dextran formers and in particular indicated that non-dextran formers should be excluded from Leuconostoc mesenteroides. The growth factor requirements support the division of the leuconostocs by other methods into six species: Leuconostoc mesenteroides, L. dextranicum, L. paramesenteroides, L. lactis, L. cremoris and L. oenos.
Two New Species of Lactobacillus Isolated from the Bovine Rumen,Lactobacillus ruminis sp.nov. and Lactobacillus vitulinus sp.nov. Two new anaerobic species of Lac-tobacillirs from the bovine rumen are described. They belong to the subgenus Tliermobacteriunz and contain nieso diaminopimelic acid in the wall peptidoglycan. The species differ from each other in being motile and non-motile, in the type of lactic acid produced and in : / $ base composition of their DNA. They have been named Lacrobacillus rumhis and L. i*iti/lims.
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